Morphological changes in rabbit corneal endothelium after surgical injuries

Masanori Miyazaki, Toshiro Tanaka, Teruo Nishida

Research output: Contribution to journalArticle

Abstract

Purpose: To understand the responses of the corneal endothelium to different types of surgery, we chronologically investigated the morphologic changes in the endothelial cells. Methods: We did a mechanical incision, epithelial ablation, and excimer laser radiation on rabbit corneas and observed the morphologic changes in the endothelial cells for up to 2 weeks after surgery under a light microscope and an electron microscope. Results: Although we observed enlarged intercellular spaces between neighboring endothelial cells, intercellular adhesion kept the cells tightly joined near their apexes immediately after each procedure. No signs of endothelial cell degeneration were observed after the procedures, but we did observe many Golgi apparati, rough-surfaced endoplasmic reticula, and secreted granules, indicating that the cells had been activated. After each procedure, the intercellular junctions and spaces required different amounts of time to return to normal. Conclusions: These results suggest that the different kinds of surgical injuries affected the corneal endothelium but that the changes were reversible.

Original languageEnglish
Pages (from-to)350-355
Number of pages6
JournalJournal of Japanese Ophthalmological Society
Volume103
Issue number5
Publication statusPublished - May 1 1999

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Corneal Endothelium
Intraoperative Complications
Endothelial Cells
Rabbits
Extracellular Space
Excimer Lasers
Intercellular Junctions
Rough Endoplasmic Reticulum
Golgi Apparatus
Cell Adhesion
Cornea
Electrons
Radiation
Light

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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Morphological changes in rabbit corneal endothelium after surgical injuries. / Miyazaki, Masanori; Tanaka, Toshiro; Nishida, Teruo.

In: Journal of Japanese Ophthalmological Society, Vol. 103, No. 5, 01.05.1999, p. 350-355.

Research output: Contribution to journalArticle

Miyazaki, Masanori ; Tanaka, Toshiro ; Nishida, Teruo. / Morphological changes in rabbit corneal endothelium after surgical injuries. In: Journal of Japanese Ophthalmological Society. 1999 ; Vol. 103, No. 5. pp. 350-355.
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