Morphological Changes in Spores during Germination in Bacillus cereus and Bacillus subtilis

Takashi Tsugukuni, Naofumi Shigemune, Motokazu Nakayama, Takahisa Miyamoto

Research output: Contribution to journalArticlepeer-review

Abstract

Processes from spore germination to outgrowth were observed in detail using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for Bacillus cereus and Bacillus subtilis. At 15 and 30 min after germination induction, SEM observation and SEM-EDX analysis of Bacillus spores prepared by freeze substitution showed that spherical structures including compounds having the same elemental ratio as that of the spore were observed on the surface of the spores. The results suggested the leakages of the cellular materials from the spores. At 360 min, B. cereus spores in outgrowth phase elongated with hemispherical structures at the end of the long side of the cells. The discoid structures with a hole (20-30 nm diameter) in the center was observed at 360 min. Confocal laser scanning microscopy after staining with fluorescence-labeled anti-spore antibodies showed that the hemispherical and discoid structures originated from the spore coat. These structures broke down after detached from the cells in outgrowth phase.

Original languageEnglish
Pages (from-to)203-213
Number of pages11
JournalBiocontrol Science
Volume25
Issue number4
DOIs
Publication statusPublished - 2020

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

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