Morphological studies of the goldfish hindgut mucosa in organ culture

Hiroshi Iida, Torao Yamamoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The morphology of the absorptive cells of the goldfish hindgut mucosa, and their capability for horseradish peroxidase (HRP) uptake, were investigated by electron microscopy after a 24-h organ culture. The columnar appearance and the fine structure of the absorptive cells were well preserved for 24 h at room temperature and 37° C with 5% CO2 in air, in all the media used in this study. Mitoses were frequently observed in the epithelium at the bottom of cultured mucosal folds, and re-epithelization was also observed in many explants. Some structural changes were, however, noted in the cultured absorptive cells, as compared with the non-cultured absorptive cells; the deep invaginations of the surface membrane between the microvilli decreased in number; supranuclear giant vacuoles were reduced in size or almost disappeared; the distributional pattern of mitochondria in the absorptive cells was altered. The HRP uptake experiments showed that the absorptive cells cultured for 24 h could still take up HRP by endocytosis and transport it, indicating that the absorptive cells maintained their capability of macromolecule uptake and transport after 24 h of culture. In addition, HRP experiments, in which reaction product was detected within numerous cytoplasmic tubules (CT), various vacuoles and CT-vacuole complexes, suggested a close relationship between CT and vacuolar system in the apical cytoplasm during endocytotic events in the absorptive cells.

Original languageEnglish
Pages (from-to)523-528
Number of pages6
JournalCell and Tissue Research
Volume238
Issue number3
DOIs
Publication statusPublished - Nov 1 1984

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Goldfish
Organ Culture Techniques
Mucous Membrane
Horseradish Peroxidase
Vacuoles
Cultured Cells
Microvilli
Endocytosis
Mitosis
Electron Microscopy
Mitochondria
Cytoplasm
Epithelium
Air
Temperature
Membranes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Morphological studies of the goldfish hindgut mucosa in organ culture. / Iida, Hiroshi; Yamamoto, Torao.

In: Cell and Tissue Research, Vol. 238, No. 3, 01.11.1984, p. 523-528.

Research output: Contribution to journalArticle

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