Morphological variety of intracellular microcolonies of Legionella species in vero cells

M. Ogawa, A. Takade, H. Miyamoto, Hatsumi Taniguchi, S. Yoshida

Research output: Contribution to journalReview article

18 Citations (Scopus)

Abstract

Intracellular microcolonies of six Legionella species growing in Vero cells showed distinctly varied morphologies. The varieties were observed by light microscopy of Gimenez-stained, Legionella-infected Vero cells and by electron microscopy (EM). Legionella pneumophila Philadelphia-1 formed needle-shaped crystal-like microcolonies. Legionella bozemanii WIGA formed microcolonies like wool balls containing filamentous cells. In EM, these organisms proliferated in endosomes, which were adjacent to swollen rough endoplasmic reticula. Legionella oakridgensis OR-10 showed serpentine chains. Many mitochondria were observed around the microcolonies. Legionella jordanis BL-540 formed spherical moss-like microcolonies which were or were not surrounded by endoplasmic membranes. Legionella feeleii WO-44C spread throughout the cytoplasm without making clusters. Legionella dumoffii Tex-KL made big clusters that spread in the cytoplasm, a portion of which was outside the endosome membranes. These different morphologies imply diversity in modes of intracellular multiplication of Legionella spp.

Original languageEnglish
Pages (from-to)557-562
Number of pages6
JournalMicrobiology and Immunology
Volume45
Issue number7
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

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Legionella
Vero Cells
Endosomes
Electron Microscopy
Cytoplasm
Bryophyta
Legionella pneumophila
Membranes
Rough Endoplasmic Reticulum
Wool
Needles
Microscopy
Mitochondria
Light

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Morphological variety of intracellular microcolonies of Legionella species in vero cells. / Ogawa, M.; Takade, A.; Miyamoto, H.; Taniguchi, Hatsumi; Yoshida, S.

In: Microbiology and Immunology, Vol. 45, No. 7, 01.01.2001, p. 557-562.

Research output: Contribution to journalReview article

Ogawa, M. ; Takade, A. ; Miyamoto, H. ; Taniguchi, Hatsumi ; Yoshida, S. / Morphological variety of intracellular microcolonies of Legionella species in vero cells. In: Microbiology and Immunology. 2001 ; Vol. 45, No. 7. pp. 557-562.
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