Morphology of sterile anthers and inheritance of cytoplasmic-genetic male sterility in zygotic seedlings of polyembryonic acid citrus

Prita Sari Dewi, Akira Wakana, Yuu Tanimoto, Yumiko Fujiwara, Kaori Sakai, Kohei Kajiwara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In acid citrus, genetic control of male sterility, an important characteristic for breeding seedless fruit cultivars, is not precisely known because of the presence of barriers such as polyembryony and a long juvenile phase. In this study, 22 crosses with 16 male-fertile acid citrus cultivars were carried out and the zygotic seedlings were grafted onto adult satsuma mandarin trees to enhance flowering. Four crosses with two monoembryonic and male-sterile citrus plants (HY16 and 'Kiyomi') were also carried out and zygotic seedlings showing precocious flowering were used to examine the inheritance of male sterility. Of the 26 crosses, 21 with six cultivars as seed parents generated male-sterile and male-fertile zygotic seedlings with various segregation ratios, whereas five crosses with 'Sudachi' as a seed parent generated only male-fertile zygotic seedlings. The sterile anthers were categorized into undeveloped and underdeveloped by their size in most progenies. The result of these crosses showed that eight cultivars with male-sterile cytoplasmic factors of 'Yuzu', lemon, or satsuma mandarin generated male-sterile zygotic seedlings, while 'Sudachi' with male-fertile cytoplasmic factors of pummelo generated only male fertile zygotic seedlings. The result also suggested that a dominant nuclear fertility-restorer gene system comprising one epistatic gene R1 and two complementary genes R2 and R3 controls the restoration of male fertility and male-sterile anther size in acid citrus plants with sterile cytoplasm (S). The complementary gene R3 is located downstream from R2, and gene R1 is epistatic to R2 and R3 genes. Genotypes (S)r1r1r2r2-- are responsible for undeveloped anthers while genotypes (S)r1r1R2-r3r3 are responsible for underdeveloped anthers. The identified genotypes for male sterility in major acid citrus cultivars will contribute to breeding seedless acid cultivars.

Original languageEnglish
Pages (from-to)203-214
Number of pages12
JournalJournal of the Japanese Society for Horticultural Science
Volume82
Issue number3
DOIs
Publication statusPublished - Jan 1 2013

Fingerprint

cytoplasmic inheritance
male fertility
anthers
Citrus
seedlings
acids
satsumas
cultivars
complementary genes
genotype
Citrus junos
polyembryony
flowering
genes
epistasis
breeding
lemons
seeds
inheritance (genetics)
cytoplasm

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Morphology of sterile anthers and inheritance of cytoplasmic-genetic male sterility in zygotic seedlings of polyembryonic acid citrus. / Dewi, Prita Sari; Wakana, Akira; Tanimoto, Yuu; Fujiwara, Yumiko; Sakai, Kaori; Kajiwara, Kohei.

In: Journal of the Japanese Society for Horticultural Science, Vol. 82, No. 3, 01.01.2013, p. 203-214.

Research output: Contribution to journalArticle

@article{1f1e50c6210f490fa262c379e9b28e50,
title = "Morphology of sterile anthers and inheritance of cytoplasmic-genetic male sterility in zygotic seedlings of polyembryonic acid citrus",
abstract = "In acid citrus, genetic control of male sterility, an important characteristic for breeding seedless fruit cultivars, is not precisely known because of the presence of barriers such as polyembryony and a long juvenile phase. In this study, 22 crosses with 16 male-fertile acid citrus cultivars were carried out and the zygotic seedlings were grafted onto adult satsuma mandarin trees to enhance flowering. Four crosses with two monoembryonic and male-sterile citrus plants (HY16 and 'Kiyomi') were also carried out and zygotic seedlings showing precocious flowering were used to examine the inheritance of male sterility. Of the 26 crosses, 21 with six cultivars as seed parents generated male-sterile and male-fertile zygotic seedlings with various segregation ratios, whereas five crosses with 'Sudachi' as a seed parent generated only male-fertile zygotic seedlings. The sterile anthers were categorized into undeveloped and underdeveloped by their size in most progenies. The result of these crosses showed that eight cultivars with male-sterile cytoplasmic factors of 'Yuzu', lemon, or satsuma mandarin generated male-sterile zygotic seedlings, while 'Sudachi' with male-fertile cytoplasmic factors of pummelo generated only male fertile zygotic seedlings. The result also suggested that a dominant nuclear fertility-restorer gene system comprising one epistatic gene R1 and two complementary genes R2 and R3 controls the restoration of male fertility and male-sterile anther size in acid citrus plants with sterile cytoplasm (S). The complementary gene R3 is located downstream from R2, and gene R1 is epistatic to R2 and R3 genes. Genotypes (S)r1r1r2r2-- are responsible for undeveloped anthers while genotypes (S)r1r1R2-r3r3 are responsible for underdeveloped anthers. The identified genotypes for male sterility in major acid citrus cultivars will contribute to breeding seedless acid cultivars.",
author = "Dewi, {Prita Sari} and Akira Wakana and Yuu Tanimoto and Yumiko Fujiwara and Kaori Sakai and Kohei Kajiwara",
year = "2013",
month = "1",
day = "1",
doi = "10.2503/jjshs1.82.203",
language = "English",
volume = "82",
pages = "203--214",
journal = "Horticulture Journal",
issn = "2189-0102",
publisher = "THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE",
number = "3",

}

TY - JOUR

T1 - Morphology of sterile anthers and inheritance of cytoplasmic-genetic male sterility in zygotic seedlings of polyembryonic acid citrus

AU - Dewi, Prita Sari

AU - Wakana, Akira

AU - Tanimoto, Yuu

AU - Fujiwara, Yumiko

AU - Sakai, Kaori

AU - Kajiwara, Kohei

PY - 2013/1/1

Y1 - 2013/1/1

N2 - In acid citrus, genetic control of male sterility, an important characteristic for breeding seedless fruit cultivars, is not precisely known because of the presence of barriers such as polyembryony and a long juvenile phase. In this study, 22 crosses with 16 male-fertile acid citrus cultivars were carried out and the zygotic seedlings were grafted onto adult satsuma mandarin trees to enhance flowering. Four crosses with two monoembryonic and male-sterile citrus plants (HY16 and 'Kiyomi') were also carried out and zygotic seedlings showing precocious flowering were used to examine the inheritance of male sterility. Of the 26 crosses, 21 with six cultivars as seed parents generated male-sterile and male-fertile zygotic seedlings with various segregation ratios, whereas five crosses with 'Sudachi' as a seed parent generated only male-fertile zygotic seedlings. The sterile anthers were categorized into undeveloped and underdeveloped by their size in most progenies. The result of these crosses showed that eight cultivars with male-sterile cytoplasmic factors of 'Yuzu', lemon, or satsuma mandarin generated male-sterile zygotic seedlings, while 'Sudachi' with male-fertile cytoplasmic factors of pummelo generated only male fertile zygotic seedlings. The result also suggested that a dominant nuclear fertility-restorer gene system comprising one epistatic gene R1 and two complementary genes R2 and R3 controls the restoration of male fertility and male-sterile anther size in acid citrus plants with sterile cytoplasm (S). The complementary gene R3 is located downstream from R2, and gene R1 is epistatic to R2 and R3 genes. Genotypes (S)r1r1r2r2-- are responsible for undeveloped anthers while genotypes (S)r1r1R2-r3r3 are responsible for underdeveloped anthers. The identified genotypes for male sterility in major acid citrus cultivars will contribute to breeding seedless acid cultivars.

AB - In acid citrus, genetic control of male sterility, an important characteristic for breeding seedless fruit cultivars, is not precisely known because of the presence of barriers such as polyembryony and a long juvenile phase. In this study, 22 crosses with 16 male-fertile acid citrus cultivars were carried out and the zygotic seedlings were grafted onto adult satsuma mandarin trees to enhance flowering. Four crosses with two monoembryonic and male-sterile citrus plants (HY16 and 'Kiyomi') were also carried out and zygotic seedlings showing precocious flowering were used to examine the inheritance of male sterility. Of the 26 crosses, 21 with six cultivars as seed parents generated male-sterile and male-fertile zygotic seedlings with various segregation ratios, whereas five crosses with 'Sudachi' as a seed parent generated only male-fertile zygotic seedlings. The sterile anthers were categorized into undeveloped and underdeveloped by their size in most progenies. The result of these crosses showed that eight cultivars with male-sterile cytoplasmic factors of 'Yuzu', lemon, or satsuma mandarin generated male-sterile zygotic seedlings, while 'Sudachi' with male-fertile cytoplasmic factors of pummelo generated only male fertile zygotic seedlings. The result also suggested that a dominant nuclear fertility-restorer gene system comprising one epistatic gene R1 and two complementary genes R2 and R3 controls the restoration of male fertility and male-sterile anther size in acid citrus plants with sterile cytoplasm (S). The complementary gene R3 is located downstream from R2, and gene R1 is epistatic to R2 and R3 genes. Genotypes (S)r1r1r2r2-- are responsible for undeveloped anthers while genotypes (S)r1r1R2-r3r3 are responsible for underdeveloped anthers. The identified genotypes for male sterility in major acid citrus cultivars will contribute to breeding seedless acid cultivars.

UR - http://www.scopus.com/inward/record.url?scp=84879287367&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879287367&partnerID=8YFLogxK

U2 - 10.2503/jjshs1.82.203

DO - 10.2503/jjshs1.82.203

M3 - Article

VL - 82

SP - 203

EP - 214

JO - Horticulture Journal

JF - Horticulture Journal

SN - 2189-0102

IS - 3

ER -