Mos1 transposon-based transformation of fish cell lines using baculoviral vectors

Masako Yokoo, Ryosuke Fujita, Yumiko Nakajima, Mamoru Yoshimizu, Hisae Kasai, Shin ichiro Asano, Hisanori Bando

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Drosophila Mos1 belongs to the mariner family of transposons, which are one of the most ubiquitous transposons among eukaryotes. We first determined nuclear transportation of the Drosophila Mos1-EGFP fusion protein in fish cell lines because it is required for a function of transposons. We next constructed recombinant baculoviral vectors harboring the Drosophila Mos1 transposon or marker genes located between Mos1 inverted repeats. The infectivity of the recombinant virus to fish cells was assessed by monitoring the expression of a fluorescent protein encoded in the viral genome. We detected transgene expression in CHSE-214, HINAE, and EPC cells, but not in GF or RTG-2 cells. In the co-infection assay of the Mos1-expressing virus and reporter gene-expressing virus, we successfully transformed CHSE-214 and HINAE cells. These results suggest that the combination of a baculovirus and Mos1 transposable element may be a tool for transgenesis in fish cells.

Original languageEnglish
Pages (from-to)18-22
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume439
Issue number1
DOIs
Publication statusPublished - Sep 13 2013
Externally publishedYes

Fingerprint

Viruses
Fish
Fishes
Genes
Cells
Cell Line
Drosophila
DNA Transposable Elements
Assays
Proteins
Fusion reactions
Gene Transfer Techniques
Baculoviridae
Viral Genome
Eukaryota
Coinfection
Transgenes
Reporter Genes
Monitoring

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mos1 transposon-based transformation of fish cell lines using baculoviral vectors. / Yokoo, Masako; Fujita, Ryosuke; Nakajima, Yumiko; Yoshimizu, Mamoru; Kasai, Hisae; Asano, Shin ichiro; Bando, Hisanori.

In: Biochemical and Biophysical Research Communications, Vol. 439, No. 1, 13.09.2013, p. 18-22.

Research output: Contribution to journalArticle

Yokoo, Masako ; Fujita, Ryosuke ; Nakajima, Yumiko ; Yoshimizu, Mamoru ; Kasai, Hisae ; Asano, Shin ichiro ; Bando, Hisanori. / Mos1 transposon-based transformation of fish cell lines using baculoviral vectors. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 439, No. 1. pp. 18-22.
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