Abstract
Using front-surface fluorometry with fura-2 and porcine aortic valvular strips, we investigated the effect of motilin, a gastrointestinal peptide, on the cytosolic Ca2+ concentration ([Ca2+]i) of endothelial cells in situ. Motilin induced a biphasic elevation of [Ca2+]i of the endothelial cells on the porcine aortic valvular strips. To investigate the physiological role of these Ca2+ transients in the endothelial cells, we determined the effect of motilin on [Ca2+]i as well as the tension of the smooth muscles in the porcine coronary strips with an intact endothelium. Motilin decreased [Ca2+]i and the tension of the coronary smooth muscle precontracted by U46619, a thromboxane A2 analogue, in an endothelium-dependent manner. In the presence of indomethacin (a cycloxygenase inhibitor), motilin induced an endothelium-dependent relaxation of the coronary strips which was partially inhibited by Nω-nitro-L-arginine (L-NNA; a NO synthase inhibitor). These results thus indicate that motilin induces Ca2+ transients of the endothelial cells while it also induces vasorelaxation, which may be mediated by both L-NNA sensitive and resistant factors that are derived from the endothelium.
| Original language | English |
|---|---|
| Pages (from-to) | 346-353 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 202 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 15 1994 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Motilin induces the endothelium-dependent relaxation of smooth muscle and the elevation of cytosolic Calcium in endothelial cells in situ. / Higuchi, Yoshihiro; Nishimura, Junji; Kanaide, Hideo.
In: Biochemical and Biophysical Research Communications, Vol. 202, No. 1, 15.07.1994, p. 346-353.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Motilin induces the endothelium-dependent relaxation of smooth muscle and the elevation of cytosolic Calcium in endothelial cells in situ
AU - Higuchi, Yoshihiro
AU - Nishimura, Junji
AU - Kanaide, Hideo
PY - 1994/7/15
Y1 - 1994/7/15
N2 - Using front-surface fluorometry with fura-2 and porcine aortic valvular strips, we investigated the effect of motilin, a gastrointestinal peptide, on the cytosolic Ca2+ concentration ([Ca2+]i) of endothelial cells in situ. Motilin induced a biphasic elevation of [Ca2+]i of the endothelial cells on the porcine aortic valvular strips. To investigate the physiological role of these Ca2+ transients in the endothelial cells, we determined the effect of motilin on [Ca2+]i as well as the tension of the smooth muscles in the porcine coronary strips with an intact endothelium. Motilin decreased [Ca2+]i and the tension of the coronary smooth muscle precontracted by U46619, a thromboxane A2 analogue, in an endothelium-dependent manner. In the presence of indomethacin (a cycloxygenase inhibitor), motilin induced an endothelium-dependent relaxation of the coronary strips which was partially inhibited by Nω-nitro-L-arginine (L-NNA; a NO synthase inhibitor). These results thus indicate that motilin induces Ca2+ transients of the endothelial cells while it also induces vasorelaxation, which may be mediated by both L-NNA sensitive and resistant factors that are derived from the endothelium.
AB - Using front-surface fluorometry with fura-2 and porcine aortic valvular strips, we investigated the effect of motilin, a gastrointestinal peptide, on the cytosolic Ca2+ concentration ([Ca2+]i) of endothelial cells in situ. Motilin induced a biphasic elevation of [Ca2+]i of the endothelial cells on the porcine aortic valvular strips. To investigate the physiological role of these Ca2+ transients in the endothelial cells, we determined the effect of motilin on [Ca2+]i as well as the tension of the smooth muscles in the porcine coronary strips with an intact endothelium. Motilin decreased [Ca2+]i and the tension of the coronary smooth muscle precontracted by U46619, a thromboxane A2 analogue, in an endothelium-dependent manner. In the presence of indomethacin (a cycloxygenase inhibitor), motilin induced an endothelium-dependent relaxation of the coronary strips which was partially inhibited by Nω-nitro-L-arginine (L-NNA; a NO synthase inhibitor). These results thus indicate that motilin induces Ca2+ transients of the endothelial cells while it also induces vasorelaxation, which may be mediated by both L-NNA sensitive and resistant factors that are derived from the endothelium.
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U2 - 10.1006/bbrc.1994.1934
DO - 10.1006/bbrc.1994.1934
M3 - Article
VL - 202
SP - 346
EP - 353
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -