Motility of Microtubules on the Inner Surface of Water-in-Oil Emulsion Droplets

Mikako Tsuji, Arif Md Rashedul Kabir, Masaki Ito, Daisuke Inoue, Kenta Kokado, Kazuki Sada, Akira Kakugo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Water-in-oil emulsion systems have recently attracted much attention in various fields. However, functionalization of water-in-oil emulsion systems, which is required for expanding their applications in industries and research, has been challenging. We now demonstrate the functionalization of a water-in-oil emulsion system by anchoring a target protein molecule. A microtubule (MT)-associated motor protein kinesin-1 was successfully anchored to the inner surface of water-in-oil emulsion droplets by employing the specific interaction of nickel-nitrilotriacetic acid-histidine tag. The MTs exhibited a gliding motion on the kinesin-functionalized inner surface of the emulsion droplets, which confirmed the success of the functionalization of the water-in-oil emulsion system. This result would be beneficial in exploring the roles of biomolecular motor systems in the cellular events that take place at the cell membrane and might also contribute to expanding the nanotechnological applications of biomolecular motors and water-in-oil emulsion systems in the future.

Original languageEnglish
Pages (from-to)12108-12113
Number of pages6
JournalLangmuir
Volume33
Issue number43
DOIs
Publication statusPublished - Oct 31 2017

Fingerprint

locomotion
Emulsions
emulsions
Oils
oils
Water
water
Kinesin
efferent nervous systems
proteins
Proteins
gliding
histidine
Cell membranes
Histidine
industries
Nickel
nickel
acids
Molecules

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Tsuji, M., Rashedul Kabir, A. M., Ito, M., Inoue, D., Kokado, K., Sada, K., & Kakugo, A. (2017). Motility of Microtubules on the Inner Surface of Water-in-Oil Emulsion Droplets. Langmuir, 33(43), 12108-12113. https://doi.org/10.1021/acs.langmuir.7b01550

Motility of Microtubules on the Inner Surface of Water-in-Oil Emulsion Droplets. / Tsuji, Mikako; Rashedul Kabir, Arif Md; Ito, Masaki; Inoue, Daisuke; Kokado, Kenta; Sada, Kazuki; Kakugo, Akira.

In: Langmuir, Vol. 33, No. 43, 31.10.2017, p. 12108-12113.

Research output: Contribution to journalArticle

Tsuji, M, Rashedul Kabir, AM, Ito, M, Inoue, D, Kokado, K, Sada, K & Kakugo, A 2017, 'Motility of Microtubules on the Inner Surface of Water-in-Oil Emulsion Droplets', Langmuir, vol. 33, no. 43, pp. 12108-12113. https://doi.org/10.1021/acs.langmuir.7b01550
Tsuji M, Rashedul Kabir AM, Ito M, Inoue D, Kokado K, Sada K et al. Motility of Microtubules on the Inner Surface of Water-in-Oil Emulsion Droplets. Langmuir. 2017 Oct 31;33(43):12108-12113. https://doi.org/10.1021/acs.langmuir.7b01550
Tsuji, Mikako ; Rashedul Kabir, Arif Md ; Ito, Masaki ; Inoue, Daisuke ; Kokado, Kenta ; Sada, Kazuki ; Kakugo, Akira. / Motility of Microtubules on the Inner Surface of Water-in-Oil Emulsion Droplets. In: Langmuir. 2017 ; Vol. 33, No. 43. pp. 12108-12113.
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