Motions and negative cooperativity between p97 domains revealed by cryo-electron microscopy and quantised elastic deformational model

Fabienne Beuron, Terence C. Flynn, Jianpeng Ma, Hisao Kondo, Xiaodong Zhang, Paul S. Freemont

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

p97, a Mg-ATPase belonging to the AAA (ATPase associated with various cellular activities) super family of proteins, has been proposed to function in two distinct cellular pathways, namely homotypic membrane fusion and ubiquitin protein degradation by utilizing differing adaptor complexes. We present the cryo-electron microscopy three-dimensional reconstruction of endogenous p97 in an AMP-PNP bound state at 24Å resolution. It reveals clear nucleotide-dependent differences when compared to our previously published "p97-ADP" reconstruction, including a striking rearrangement of N domains and a positional change of the two ATPase domains, D1 and D2, with respect to each other. The docking of the X-ray structure of N-D1 domains in an ADP bound state indicates that an upward repositioning of N domain is necessary to accommodate the cryo-EM map of "p97-AMP-PNP", suggesting a change in the orientation of N domains upon nucleotide hydrolysis. Furthermore, computational analysis of the deformational motions of p97, performed on the cryo-EM density map and the atomic structure of the N-D1 domains independently, shows the existence of a negative cooperativity between the D1 and D2 rings and the flexibility of the N domains. Together these results allow the identification of functionally important features that offer molecular insights into the dynamics of the proposed p97 chaperone function.

Original languageEnglish
Pages (from-to)619-629
Number of pages11
JournalJournal of Molecular Biology
Volume327
Issue number3
DOIs
Publication statusPublished - Mar 28 2003
Externally publishedYes

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Cryoelectron Microscopy
Adenylyl Imidodiphosphate
Adenosine Triphosphatases
Adenosine Diphosphate
Nucleotides
Membrane Fusion Proteins
Ubiquitin
Proteolysis
Hydrolysis
X-Rays
Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Motions and negative cooperativity between p97 domains revealed by cryo-electron microscopy and quantised elastic deformational model. / Beuron, Fabienne; Flynn, Terence C.; Ma, Jianpeng; Kondo, Hisao; Zhang, Xiaodong; Freemont, Paul S.

In: Journal of Molecular Biology, Vol. 327, No. 3, 28.03.2003, p. 619-629.

Research output: Contribution to journalArticle

Beuron, Fabienne ; Flynn, Terence C. ; Ma, Jianpeng ; Kondo, Hisao ; Zhang, Xiaodong ; Freemont, Paul S. / Motions and negative cooperativity between p97 domains revealed by cryo-electron microscopy and quantised elastic deformational model. In: Journal of Molecular Biology. 2003 ; Vol. 327, No. 3. pp. 619-629.
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