Mouse 3-phosphoglycerate dehydrogenase gene: Genomic organization, chromosomal localization, and promoter analysis

Junya Mitoma, Shigeki Furuya, Motohiro Shimizu, Yoko Shinoda, Kazuyuki Yoshida, Norihiro Azuma, Hideyuki Tanaka, Yasuo Suzuki, Yoshio Hirabayashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

D-3-Phosphoglycerate dehydrogenase (Phgdh; EC 1.1.1.95) is the first committed enzyme of L-serine biosynthesis in the phosphorylated pathway. We have recently demonstrated that, in developing and mature brain, expression of Phgdh is highly regulated in a cell lineage-specific manner, mainly in neuroepithelial stem cells, radial glia, and astrocytes (J. Neurosci. 21 (2001) 7691; Arch. Histol. Cytol. 66 (2003) 109). To gain insight into the regulatory mechanism of Phgdh expression, we have isolated a mouse genomic clone that contains the entire mouse Phgdh gene. Structural analysis demonstrated that the Phgdh gene spans approximately 27 kilobases (kb) in length and comprises 12 exons with 11 intervening introns. Using fluorescent in situ hybridization (FISH), we mapped the gene to mouse chromosome 3, region F2-F3. Analysis of a 1.8 kb fragment of the 5′-flanking region showed that the classical TATA-box motif near transcription initiation sites was absent. Instead, a GC-rich proximal region containing a potential Sp1 recognition sequence was present; this region is conserved in mouse, rat, and human counterparts. Transient transfection analysis revealed that the cis-acting elements necessary for basal transcription of Phgdh are contained within the -196/+4 proximal sequence of the promoter, in which the conserved Sp1 recognition sites play an important role for basal promoter activity.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalGene
Volume334
Issue number1-2
DOIs
Publication statusPublished - Jun 9 2004

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Phosphoglycerate Dehydrogenase
Genes
GC Rich Sequence
Neuroepithelial Cells
TATA Box
Chromosomes, Human, Pair 3
5' Flanking Region
Transcription Initiation Site
Cell Lineage
Fluorescence In Situ Hybridization
Neuroglia
Astrocytes
Introns
Serine
Transfection
Exons
Stem Cells
Clone Cells
Brain
Enzymes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Mouse 3-phosphoglycerate dehydrogenase gene : Genomic organization, chromosomal localization, and promoter analysis. / Mitoma, Junya; Furuya, Shigeki; Shimizu, Motohiro; Shinoda, Yoko; Yoshida, Kazuyuki; Azuma, Norihiro; Tanaka, Hideyuki; Suzuki, Yasuo; Hirabayashi, Yoshio.

In: Gene, Vol. 334, No. 1-2, 09.06.2004, p. 15-22.

Research output: Contribution to journalArticle

Mitoma, J, Furuya, S, Shimizu, M, Shinoda, Y, Yoshida, K, Azuma, N, Tanaka, H, Suzuki, Y & Hirabayashi, Y 2004, 'Mouse 3-phosphoglycerate dehydrogenase gene: Genomic organization, chromosomal localization, and promoter analysis', Gene, vol. 334, no. 1-2, pp. 15-22. https://doi.org/10.1016/j.gene.2004.03.015
Mitoma, Junya ; Furuya, Shigeki ; Shimizu, Motohiro ; Shinoda, Yoko ; Yoshida, Kazuyuki ; Azuma, Norihiro ; Tanaka, Hideyuki ; Suzuki, Yasuo ; Hirabayashi, Yoshio. / Mouse 3-phosphoglycerate dehydrogenase gene : Genomic organization, chromosomal localization, and promoter analysis. In: Gene. 2004 ; Vol. 334, No. 1-2. pp. 15-22.
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