Abstract
D-3-Phosphoglycerate dehydrogenase (Phgdh; EC 1.1.1.95) is the first committed enzyme of L-serine biosynthesis in the phosphorylated pathway. We have recently demonstrated that, in developing and mature brain, expression of Phgdh is highly regulated in a cell lineage-specific manner, mainly in neuroepithelial stem cells, radial glia, and astrocytes (J. Neurosci. 21 (2001) 7691; Arch. Histol. Cytol. 66 (2003) 109). To gain insight into the regulatory mechanism of Phgdh expression, we have isolated a mouse genomic clone that contains the entire mouse Phgdh gene. Structural analysis demonstrated that the Phgdh gene spans approximately 27 kilobases (kb) in length and comprises 12 exons with 11 intervening introns. Using fluorescent in situ hybridization (FISH), we mapped the gene to mouse chromosome 3, region F2-F3. Analysis of a 1.8 kb fragment of the 5′-flanking region showed that the classical TATA-box motif near transcription initiation sites was absent. Instead, a GC-rich proximal region containing a potential Sp1 recognition sequence was present; this region is conserved in mouse, rat, and human counterparts. Transient transfection analysis revealed that the cis-acting elements necessary for basal transcription of Phgdh are contained within the -196/+4 proximal sequence of the promoter, in which the conserved Sp1 recognition sites play an important role for basal promoter activity.
| Original language | English |
|---|---|
| Pages (from-to) | 15-22 |
| Number of pages | 8 |
| Journal | Gene |
| Volume | 334 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Jun 9 2004 |
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All Science Journal Classification (ASJC) codes
- Genetics
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Mouse 3-phosphoglycerate dehydrogenase gene : Genomic organization, chromosomal localization, and promoter analysis. / Mitoma, Junya; Furuya, Shigeki; Shimizu, Motohiro; Shinoda, Yoko; Yoshida, Kazuyuki; Azuma, Norihiro; Tanaka, Hideyuki; Suzuki, Yasuo; Hirabayashi, Yoshio.
In: Gene, Vol. 334, No. 1-2, 09.06.2004, p. 15-22.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mouse 3-phosphoglycerate dehydrogenase gene
T2 - Genomic organization, chromosomal localization, and promoter analysis
AU - Mitoma, Junya
AU - Furuya, Shigeki
AU - Shimizu, Motohiro
AU - Shinoda, Yoko
AU - Yoshida, Kazuyuki
AU - Azuma, Norihiro
AU - Tanaka, Hideyuki
AU - Suzuki, Yasuo
AU - Hirabayashi, Yoshio
PY - 2004/6/9
Y1 - 2004/6/9
N2 - D-3-Phosphoglycerate dehydrogenase (Phgdh; EC 1.1.1.95) is the first committed enzyme of L-serine biosynthesis in the phosphorylated pathway. We have recently demonstrated that, in developing and mature brain, expression of Phgdh is highly regulated in a cell lineage-specific manner, mainly in neuroepithelial stem cells, radial glia, and astrocytes (J. Neurosci. 21 (2001) 7691; Arch. Histol. Cytol. 66 (2003) 109). To gain insight into the regulatory mechanism of Phgdh expression, we have isolated a mouse genomic clone that contains the entire mouse Phgdh gene. Structural analysis demonstrated that the Phgdh gene spans approximately 27 kilobases (kb) in length and comprises 12 exons with 11 intervening introns. Using fluorescent in situ hybridization (FISH), we mapped the gene to mouse chromosome 3, region F2-F3. Analysis of a 1.8 kb fragment of the 5′-flanking region showed that the classical TATA-box motif near transcription initiation sites was absent. Instead, a GC-rich proximal region containing a potential Sp1 recognition sequence was present; this region is conserved in mouse, rat, and human counterparts. Transient transfection analysis revealed that the cis-acting elements necessary for basal transcription of Phgdh are contained within the -196/+4 proximal sequence of the promoter, in which the conserved Sp1 recognition sites play an important role for basal promoter activity.
AB - D-3-Phosphoglycerate dehydrogenase (Phgdh; EC 1.1.1.95) is the first committed enzyme of L-serine biosynthesis in the phosphorylated pathway. We have recently demonstrated that, in developing and mature brain, expression of Phgdh is highly regulated in a cell lineage-specific manner, mainly in neuroepithelial stem cells, radial glia, and astrocytes (J. Neurosci. 21 (2001) 7691; Arch. Histol. Cytol. 66 (2003) 109). To gain insight into the regulatory mechanism of Phgdh expression, we have isolated a mouse genomic clone that contains the entire mouse Phgdh gene. Structural analysis demonstrated that the Phgdh gene spans approximately 27 kilobases (kb) in length and comprises 12 exons with 11 intervening introns. Using fluorescent in situ hybridization (FISH), we mapped the gene to mouse chromosome 3, region F2-F3. Analysis of a 1.8 kb fragment of the 5′-flanking region showed that the classical TATA-box motif near transcription initiation sites was absent. Instead, a GC-rich proximal region containing a potential Sp1 recognition sequence was present; this region is conserved in mouse, rat, and human counterparts. Transient transfection analysis revealed that the cis-acting elements necessary for basal transcription of Phgdh are contained within the -196/+4 proximal sequence of the promoter, in which the conserved Sp1 recognition sites play an important role for basal promoter activity.
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UR - http://www.scopus.com/inward/citedby.url?scp=2542417785&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2004.03.015
DO - 10.1016/j.gene.2004.03.015
M3 - Article
C2 - 15256251
AN - SCOPUS:2542417785
VL - 334
SP - 15
EP - 22
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -