Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative y

Tatsuya Nishihara, Hiroshi Nonaka, Tatsuya Naganuma, Kazuhiro Ichikawa, Shinsuke Sando

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    We developed a promising candidate for a hypersensitive magnetic resonance (MR) reporter protein. The reporter protein is comprised of mouse lactate dehydrogenase-X (mLDH-X) and a hyperpolarized pyruvic acid derivative, substrate Y that gives no false-positive signals by endogenous human lactate dehydrogenases (hLDHs). The X-Y pair shows high MR sensitivity and sufficient reaction kinetics, thus enabling direct detection of reporter protein X in crude biological mixtures by monitoring the 13C NMR signal of hyperpolarized product Y r.

    Original languageEnglish
    Pages (from-to)800-806
    Number of pages7
    JournalChemical Science
    Volume3
    Issue number3
    DOIs
    Publication statusPublished - Mar 2012

    All Science Journal Classification (ASJC) codes

    • Chemistry(all)

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