Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative y

Tatsuya Nishihara, Hiroshi Nonaka, Tatsuya Naganuma, Kazuhiro Ichikawa, Shinsuke Sando

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We developed a promising candidate for a hypersensitive magnetic resonance (MR) reporter protein. The reporter protein is comprised of mouse lactate dehydrogenase-X (mLDH-X) and a hyperpolarized pyruvic acid derivative, substrate Y that gives no false-positive signals by endogenous human lactate dehydrogenases (hLDHs). The X-Y pair shows high MR sensitivity and sufficient reaction kinetics, thus enabling direct detection of reporter protein X in crude biological mixtures by monitoring the 13C NMR signal of hyperpolarized product Y r.

Original languageEnglish
Pages (from-to)800-806
Number of pages7
JournalChemical Science
Volume3
Issue number3
DOIs
Publication statusPublished - Mar 1 2012

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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