Mouse polycomb group gene Cbx2 promotes osteoblastic but suppresses adipogenic differentiation in postnatal long bones

Yuko Katoh-Fukui, Takashi Baba, Tetsuya Sato, Hiroyuki Otake, Yuko Nagakui-Noguchi, Miyuki Shindo, Mikita Suyama, Yasuyuki Ohkawa, Hideki Tsumura, Ken-Ichirou Morohashi, Maki Fukami

Research output: Contribution to journalArticle

Abstract

A set of key developmental genes is essential for skeletal growth from multipotent progenitor cells at weaning. Polycomb group proteins, which regulate such genes contributes to the cell lineage commitment and subsequent differentiation via epigenetic chromatin modification and remodeling. However, it is unclear which cell lineage and gene sets are targeted by polycomb proteins during skeletal growth. We now report that mice deficient in a polycomb group gene Cbx2cterm/cterm exhibited skeletal hypoplasia in the tibia, femur, and cranium. Long bone cavities in these mice contained fewer multipotent mesenchymal stromal cells. RNA-sequencing of bone marrow cells showed downregulation and upregulation of osteoblastic and adipogenic genes, respectively. Furthermore, the expression levels of genes specifically expressed in B-cell precursors were decreased. Forced expression of Cbx2 in Cbx2cterm/cterm bone marrow stromal cell recovered fibroblastic colony formation and suppressed adipogenic differentiation. Collectively, our results suggest that Cbx2 controls the maintenance and adipogenic differentiation of mesenchymal stromal cells in the bone marrow.

LanguageEnglish
Pages219-231
Number of pages13
JournalBone
Volume120
DOIs
Publication statusPublished - Mar 1 2019

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Adipocytes
Bone Marrow
Mesenchymal Stromal Cells
Bone and Bones
Cell Lineage
Growth
Genes
Polycomb-Group Proteins
RNA Sequence Analysis
Developmental Genes
Chromatin Assembly and Disassembly
B-Lymphoid Precursor Cells
Weaning
Tibia
Epigenomics
Skull
Bone Marrow Cells
Femur
Up-Regulation
Stem Cells

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Mouse polycomb group gene Cbx2 promotes osteoblastic but suppresses adipogenic differentiation in postnatal long bones. / Katoh-Fukui, Yuko; Baba, Takashi; Sato, Tetsuya; Otake, Hiroyuki; Nagakui-Noguchi, Yuko; Shindo, Miyuki; Suyama, Mikita; Ohkawa, Yasuyuki; Tsumura, Hideki; Morohashi, Ken-Ichirou; Fukami, Maki.

In: Bone, Vol. 120, 01.03.2019, p. 219-231.

Research output: Contribution to journalArticle

Katoh-Fukui, Yuko ; Baba, Takashi ; Sato, Tetsuya ; Otake, Hiroyuki ; Nagakui-Noguchi, Yuko ; Shindo, Miyuki ; Suyama, Mikita ; Ohkawa, Yasuyuki ; Tsumura, Hideki ; Morohashi, Ken-Ichirou ; Fukami, Maki. / Mouse polycomb group gene Cbx2 promotes osteoblastic but suppresses adipogenic differentiation in postnatal long bones. In: Bone. 2019 ; Vol. 120. pp. 219-231.
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