TY - JOUR
T1 - mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules
AU - Masuda, Satohiro
AU - Saito, Hideyuki
AU - Nonoguchi, Hiroshi
AU - Tomita, Kimio
AU - Inui, Ken Ichi
N1 - Funding Information:
This work was supported by a Grant-in Aid for Scientific Research (B) and a Grant-in Aid for Scientific Research on Priority Areas of `Channel-Transporter Correlation' from the Ministry of Education, Science, and Culture of Japan.
PY - 1997/4/28
Y1 - 1997/4/28
N2 - OAT-K1, a renal organic anion transporter, which mediates methotrexate uptake, was analyzed for mRNA distribution along microdissected nephron segments and the immunolozalization in isolated plasma membranes from rat kidney. By using a reverse transcription-coupled PCR, OAT-K1 mRNA was detected predominantly in the superficial and juxtamedullary proximal straight tubules. Western blotting with antiserum for OAT-K1 revealed that the transporter protein with the apparent molecular mass of 40 kDa was expressed exclusively in the brush-border membranes from rat kidney. These findings suggest that the OAT-K1 is localized in the brush-border membranes of the renal proximal straight tubules.
AB - OAT-K1, a renal organic anion transporter, which mediates methotrexate uptake, was analyzed for mRNA distribution along microdissected nephron segments and the immunolozalization in isolated plasma membranes from rat kidney. By using a reverse transcription-coupled PCR, OAT-K1 mRNA was detected predominantly in the superficial and juxtamedullary proximal straight tubules. Western blotting with antiserum for OAT-K1 revealed that the transporter protein with the apparent molecular mass of 40 kDa was expressed exclusively in the brush-border membranes from rat kidney. These findings suggest that the OAT-K1 is localized in the brush-border membranes of the renal proximal straight tubules.
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U2 - 10.1016/S0014-5793(97)00314-1
DO - 10.1016/S0014-5793(97)00314-1
M3 - Article
C2 - 9166885
AN - SCOPUS:0031589135
SN - 0014-5793
VL - 407
SP - 127
EP - 131
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -