mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules

Satohiro Masuda, Hideyuki Saito, Hiroshi Nonoguchi, Kimio Tomita, Ken Ichi Inui

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

OAT-K1, a renal organic anion transporter, which mediates methotrexate uptake, was analyzed for mRNA distribution along microdissected nephron segments and the immunolozalization in isolated plasma membranes from rat kidney. By using a reverse transcription-coupled PCR, OAT-K1 mRNA was detected predominantly in the superficial and juxtamedullary proximal straight tubules. Western blotting with antiserum for OAT-K1 revealed that the transporter protein with the apparent molecular mass of 40 kDa was expressed exclusively in the brush-border membranes from rat kidney. These findings suggest that the OAT-K1 is localized in the brush-border membranes of the renal proximal straight tubules.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalFEBS Letters
Volume407
Issue number2
DOIs
Publication statusPublished - Apr 28 1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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