mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules

Satohiro Masuda, Hideyuki Saito, Hiroshi Nonoguchi, Kimio Tomita, Ken Ichi Inui

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

OAT-K1, a renal organic anion transporter, which mediates methotrexate uptake, was analyzed for mRNA distribution along microdissected nephron segments and the immunolozalization in isolated plasma membranes from rat kidney. By using a reverse transcription-coupled PCR, OAT-K1 mRNA was detected predominantly in the superficial and juxtamedullary proximal straight tubules. Western blotting with antiserum for OAT-K1 revealed that the transporter protein with the apparent molecular mass of 40 kDa was expressed exclusively in the brush-border membranes from rat kidney. These findings suggest that the OAT-K1 is localized in the brush-border membranes of the renal proximal straight tubules.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalFEBS Letters
Volume407
Issue number2
DOIs
Publication statusPublished - Apr 28 1997

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Organic Anion Transporters
Brushes
Rats
Microvilli
Membranes
Kidney
Messenger RNA
Molecular mass
Transcription
Cell membranes
Methotrexate
Immune Sera
Proximal Kidney Tubule
Nephrons
Reverse Transcription
Western Blotting
Cell Membrane
Polymerase Chain Reaction
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules. / Masuda, Satohiro; Saito, Hideyuki; Nonoguchi, Hiroshi; Tomita, Kimio; Inui, Ken Ichi.

In: FEBS Letters, Vol. 407, No. 2, 28.04.1997, p. 127-131.

Research output: Contribution to journalArticle

Masuda, Satohiro ; Saito, Hideyuki ; Nonoguchi, Hiroshi ; Tomita, Kimio ; Inui, Ken Ichi. / mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules. In: FEBS Letters. 1997 ; Vol. 407, No. 2. pp. 127-131.
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