Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004

Tamie Noda, Koichi Murakami, Tetsuo Asai, Yoshiki Etoh, Tomoe Ishihara, Toshiro Kuroki, Kazumi Horikawa, Shuji Fujimoto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in the dominant lineage before and after this event remain unknown. This study determined S. Enteritidis lineages before and after this pandemic event in Japan using multilocus sequence typing (MLST). Thirty S. Enteritidis strains were collected in Japan between 1973 and 2004, consisting of 27 human strains from individual episodes, a bovine strain, a liquid egg strain and an eggshell strain. Strains showed nine phage types and 17 pulsed-field profiles with pulsed-field gel electrophoresis. All strains had homologous type 11 sequences without any nucleotide differences in seven housekeeping genes. These MLST results suggest that S. Enteritidis with the diversities revealed by phage typing and pulsed-field profiling has a highly clonal population. Although type 11 S. Enteritidis may exhibit both pleiotropic surface structure and pulsed-field type variation, it is likely to be a stable lineage derived from an ancestor before the 1980s and/or 1990s pandemic in Japan.

Original languageEnglish
Article number38
JournalActa Veterinaria Scandinavica
Volume53
Issue number1
DOIs
Publication statusPublished - Jun 15 2011

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Salmonella enterica
Salmonella Enteritidis
Japan
Pandemics
Multilocus Sequence Typing
serotypes
pandemic
Egg Shell
Bacteriophage Typing
bacteriophages
Pulsed Field Gel Electrophoresis
Essential Genes
Sequence Homology
Bacteriophages
Ovum
Nucleotides
pulsed-field gel electrophoresis
egg shell
Serogroup
multilocus sequence typing

All Science Journal Classification (ASJC) codes

  • veterinary(all)

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Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004. / Noda, Tamie; Murakami, Koichi; Asai, Tetsuo; Etoh, Yoshiki; Ishihara, Tomoe; Kuroki, Toshiro; Horikawa, Kazumi; Fujimoto, Shuji.

In: Acta Veterinaria Scandinavica, Vol. 53, No. 1, 38, 15.06.2011.

Research output: Contribution to journalArticle

Noda, Tamie ; Murakami, Koichi ; Asai, Tetsuo ; Etoh, Yoshiki ; Ishihara, Tomoe ; Kuroki, Toshiro ; Horikawa, Kazumi ; Fujimoto, Shuji. / Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004. In: Acta Veterinaria Scandinavica. 2011 ; Vol. 53, No. 1.
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