Abstract
We describe the preparation of well-defined multicompartment micelles from polybutadiene-block-poly(1-methyl-2-vinyl pyridinium methyl sulfate)-block- poly(methacrylic acid) (BVqMAA) triblock terpolymers and their use as advanced drug delivery systems for photodynamic therapy (PDT). A porphyrazine derivative was incorporated into the hydrophobic core during self-assembly and served as a model drug and fluorescent probe at the same time. The initial micellar corona is formed by negatively charged PMAA and could be gradually changed to poly(ethylene glycol) (PEG) in a controlled fashion through interpolyelectrolyte complex formation of PMAA with positively charged poly(ethylene glycol)-block-poly(l-lysine) (PLL-b-PEG) diblock copolymers. At high degrees of PEGylation, a compartmentalized micellar corona was observed, with a stable bottlebrush-on-sphere morphology as demonstrated by cryo-TEM measurements. By in vitro cellular experiments, we confirmed that the porphyrazine-loaded micelles were PDT-active against A549 cells. The corona composition strongly influenced their in vitro PDT activity, which decreased with increasing PEGylation, correlating with the cellular uptake of the micelles. Also, a PEGylation-dependent influence on the in vivo blood circulation and tumor accumulation was found. Fully PEGylated micelles were detected for up to 24 h in the bloodstream and accumulated in solid subcutaneous A549 tumors, while non-or only partially PEGylated micelles were rapidly cleared and did not accumulate in tumor tissue. Efficient tumor growth suppression was shown for fully PEGylated micelles up to 20 days, demonstrating PDT efficacy in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 1161-1172 |
| Number of pages | 12 |
| Journal | ACS nano |
| Volume | 8 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 25 2014 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)
Cite this
Multicompartment micelles with adjustable poly(ethylene glycol) shell for efficient in vivo photodynamic therapy. / Synatschke, Christopher V.; Nomoto, Takahiro; Cabral, Horacio; Förtsch, Melanie; Toh, Kazuko; Matsumoto, Yu; Miyazaki, Kozo; Hanisch, Andreas; Schacher, Felix H.; Kishimura, Akihiro; Nishiyama, Nobuhiro; Müller, Axel H.E.; Kataoka, Kazunori.
In: ACS nano, Vol. 8, No. 2, 25.02.2014, p. 1161-1172.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Multicompartment micelles with adjustable poly(ethylene glycol) shell for efficient in vivo photodynamic therapy
AU - Synatschke, Christopher V.
AU - Nomoto, Takahiro
AU - Cabral, Horacio
AU - Förtsch, Melanie
AU - Toh, Kazuko
AU - Matsumoto, Yu
AU - Miyazaki, Kozo
AU - Hanisch, Andreas
AU - Schacher, Felix H.
AU - Kishimura, Akihiro
AU - Nishiyama, Nobuhiro
AU - Müller, Axel H.E.
AU - Kataoka, Kazunori
PY - 2014/2/25
Y1 - 2014/2/25
N2 - We describe the preparation of well-defined multicompartment micelles from polybutadiene-block-poly(1-methyl-2-vinyl pyridinium methyl sulfate)-block- poly(methacrylic acid) (BVqMAA) triblock terpolymers and their use as advanced drug delivery systems for photodynamic therapy (PDT). A porphyrazine derivative was incorporated into the hydrophobic core during self-assembly and served as a model drug and fluorescent probe at the same time. The initial micellar corona is formed by negatively charged PMAA and could be gradually changed to poly(ethylene glycol) (PEG) in a controlled fashion through interpolyelectrolyte complex formation of PMAA with positively charged poly(ethylene glycol)-block-poly(l-lysine) (PLL-b-PEG) diblock copolymers. At high degrees of PEGylation, a compartmentalized micellar corona was observed, with a stable bottlebrush-on-sphere morphology as demonstrated by cryo-TEM measurements. By in vitro cellular experiments, we confirmed that the porphyrazine-loaded micelles were PDT-active against A549 cells. The corona composition strongly influenced their in vitro PDT activity, which decreased with increasing PEGylation, correlating with the cellular uptake of the micelles. Also, a PEGylation-dependent influence on the in vivo blood circulation and tumor accumulation was found. Fully PEGylated micelles were detected for up to 24 h in the bloodstream and accumulated in solid subcutaneous A549 tumors, while non-or only partially PEGylated micelles were rapidly cleared and did not accumulate in tumor tissue. Efficient tumor growth suppression was shown for fully PEGylated micelles up to 20 days, demonstrating PDT efficacy in vivo.
AB - We describe the preparation of well-defined multicompartment micelles from polybutadiene-block-poly(1-methyl-2-vinyl pyridinium methyl sulfate)-block- poly(methacrylic acid) (BVqMAA) triblock terpolymers and their use as advanced drug delivery systems for photodynamic therapy (PDT). A porphyrazine derivative was incorporated into the hydrophobic core during self-assembly and served as a model drug and fluorescent probe at the same time. The initial micellar corona is formed by negatively charged PMAA and could be gradually changed to poly(ethylene glycol) (PEG) in a controlled fashion through interpolyelectrolyte complex formation of PMAA with positively charged poly(ethylene glycol)-block-poly(l-lysine) (PLL-b-PEG) diblock copolymers. At high degrees of PEGylation, a compartmentalized micellar corona was observed, with a stable bottlebrush-on-sphere morphology as demonstrated by cryo-TEM measurements. By in vitro cellular experiments, we confirmed that the porphyrazine-loaded micelles were PDT-active against A549 cells. The corona composition strongly influenced their in vitro PDT activity, which decreased with increasing PEGylation, correlating with the cellular uptake of the micelles. Also, a PEGylation-dependent influence on the in vivo blood circulation and tumor accumulation was found. Fully PEGylated micelles were detected for up to 24 h in the bloodstream and accumulated in solid subcutaneous A549 tumors, while non-or only partially PEGylated micelles were rapidly cleared and did not accumulate in tumor tissue. Efficient tumor growth suppression was shown for fully PEGylated micelles up to 20 days, demonstrating PDT efficacy in vivo.
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UR - http://www.scopus.com/inward/citedby.url?scp=84894627818&partnerID=8YFLogxK
U2 - 10.1021/nn4028294
DO - 10.1021/nn4028294
M3 - Article
C2 - 24386876
AN - SCOPUS:84894627818
VL - 8
SP - 1161
EP - 1172
JO - ACS Nano
JF - ACS Nano
SN - 1936-0851
IS - 2
ER -