Multifunctional flow-injection biosensor for the simultaneous measurement of creatinine, glucose, and urea

Chang Sheng Rui, Hiroaki I. Ogawa, Kenji Sonomoto, Yasuhiko Kato, Kenji Sonomoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An amperometric flow-injection biosensor system was developed for the simultaneous measurement of creatinine, glucose, and urea, with a single sample injection and one detector. The principle for the amperometric detection of urea and creatinine was based on coupled reactions of three sequentially aligned enzyme reactors, urease or creatinine deiminase/glutamate dehydrogenase/L-glutamate oxidase. Inclusion of a split point and two confluence points between the injector and detector (oxygen electrode) resulted in a flow-injection system composed of three channels. Triple peak recording was obtained by putting two delay coils of different lengths at the channels involving urease and glucose oxidase. The system gave linear calibrations for creatinine, glucose, and urea in the ranges of 0.2–5, 0.2–10, and 0.5–20mM, respectively. The assay procedure was simple and one run was completed within 4 min. The system was reproducible within 5–8% of relative standard deviation.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume57
Issue number2
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

Fingerprint

Biosensing Techniques
Biosensors
Urea
Creatinine
Urease
L-glutamate oxidase
creatinine deiminase
Glucose
Injections
Detectors
Glutamate Dehydrogenase
Glucose Oxidase
Calibration
Assays
Electrodes
Oxygen
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Multifunctional flow-injection biosensor for the simultaneous measurement of creatinine, glucose, and urea. / Rui, Chang Sheng; Ogawa, Hiroaki I.; Sonomoto, Kenji; Kato, Yasuhiko; Sonomoto, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 57, No. 2, 01.01.1993, p. 191-194.

Research output: Contribution to journalArticle

Rui, Chang Sheng ; Ogawa, Hiroaki I. ; Sonomoto, Kenji ; Kato, Yasuhiko ; Sonomoto, Kenji. / Multifunctional flow-injection biosensor for the simultaneous measurement of creatinine, glucose, and urea. In: Bioscience, Biotechnology and Biochemistry. 1993 ; Vol. 57, No. 2. pp. 191-194.
@article{e48949845c0441bf8db69baa559c4d1b,
title = "Multifunctional flow-injection biosensor for the simultaneous measurement of creatinine, glucose, and urea",
abstract = "An amperometric flow-injection biosensor system was developed for the simultaneous measurement of creatinine, glucose, and urea, with a single sample injection and one detector. The principle for the amperometric detection of urea and creatinine was based on coupled reactions of three sequentially aligned enzyme reactors, urease or creatinine deiminase/glutamate dehydrogenase/L-glutamate oxidase. Inclusion of a split point and two confluence points between the injector and detector (oxygen electrode) resulted in a flow-injection system composed of three channels. Triple peak recording was obtained by putting two delay coils of different lengths at the channels involving urease and glucose oxidase. The system gave linear calibrations for creatinine, glucose, and urea in the ranges of 0.2–5, 0.2–10, and 0.5–20mM, respectively. The assay procedure was simple and one run was completed within 4 min. The system was reproducible within 5–8{\%} of relative standard deviation.",
author = "Rui, {Chang Sheng} and Ogawa, {Hiroaki I.} and Kenji Sonomoto and Yasuhiko Kato and Kenji Sonomoto",
year = "1993",
month = "1",
day = "1",
doi = "10.1271/bbb.57.191",
language = "English",
volume = "57",
pages = "191--194",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "2",

}

TY - JOUR

T1 - Multifunctional flow-injection biosensor for the simultaneous measurement of creatinine, glucose, and urea

AU - Rui, Chang Sheng

AU - Ogawa, Hiroaki I.

AU - Sonomoto, Kenji

AU - Kato, Yasuhiko

AU - Sonomoto, Kenji

PY - 1993/1/1

Y1 - 1993/1/1

N2 - An amperometric flow-injection biosensor system was developed for the simultaneous measurement of creatinine, glucose, and urea, with a single sample injection and one detector. The principle for the amperometric detection of urea and creatinine was based on coupled reactions of three sequentially aligned enzyme reactors, urease or creatinine deiminase/glutamate dehydrogenase/L-glutamate oxidase. Inclusion of a split point and two confluence points between the injector and detector (oxygen electrode) resulted in a flow-injection system composed of three channels. Triple peak recording was obtained by putting two delay coils of different lengths at the channels involving urease and glucose oxidase. The system gave linear calibrations for creatinine, glucose, and urea in the ranges of 0.2–5, 0.2–10, and 0.5–20mM, respectively. The assay procedure was simple and one run was completed within 4 min. The system was reproducible within 5–8% of relative standard deviation.

AB - An amperometric flow-injection biosensor system was developed for the simultaneous measurement of creatinine, glucose, and urea, with a single sample injection and one detector. The principle for the amperometric detection of urea and creatinine was based on coupled reactions of three sequentially aligned enzyme reactors, urease or creatinine deiminase/glutamate dehydrogenase/L-glutamate oxidase. Inclusion of a split point and two confluence points between the injector and detector (oxygen electrode) resulted in a flow-injection system composed of three channels. Triple peak recording was obtained by putting two delay coils of different lengths at the channels involving urease and glucose oxidase. The system gave linear calibrations for creatinine, glucose, and urea in the ranges of 0.2–5, 0.2–10, and 0.5–20mM, respectively. The assay procedure was simple and one run was completed within 4 min. The system was reproducible within 5–8% of relative standard deviation.

UR - http://www.scopus.com/inward/record.url?scp=0000071223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000071223&partnerID=8YFLogxK

U2 - 10.1271/bbb.57.191

DO - 10.1271/bbb.57.191

M3 - Article

VL - 57

SP - 191

EP - 194

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 2

ER -