Multimodal imaging of living cells with multiplex coherent anti-stokes Raman scattering (CARS), third-order sum frequency generation (TSFG) and two-photon excitation fluorescence (TPEF) using a nanosecond white-light laser source

Hiroki Segawa, Masanari Okuno, Philippe Leproux, Vincent Couderc, Takeaki Ozawa, Hideaki Kano

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The subnanosecond "white-light laser" source has been applied to multimodal, multiphoton, and multiplex spectroscopic imaging (M 3 spectroscopic imaging) with coherent anti-Stokes Raman scattering (CARS), third-order sum frequency generation (TSFG), and two-photon excitation fluorescence (TPEF). As the proof-of-principle experiment, we performed simultaneous imaging of polystyrene beads with TSFG and TPEF. This technique is then applied to live cell imaging. Mouse L929 fibroblastic cells are clearly visualized by CARS, TSFG, and TPEF processes. M 3 spectroscopic imaging provides various and unique cellular information with different image contrast based on each multiphoton process.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
Journalanalytical sciences
Volume31
Issue number4
DOIs
Publication statusPublished - 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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