TY - GEN
T1 - Multiphase-laden gas-liquid interface injection for the versatile gene transfer
AU - Kuriki, H.
AU - Takasawa, S.
AU - Iwabuchi, M.
AU - Ohsumi, K.
AU - Suzuki, T.
AU - Higashiyama, T.
AU - Sakuma, S.
AU - Arai, F.
AU - Yamanishi, Y.
PY - 2013
Y1 - 2013
N2 - We have successfully carried out gene transfer using electrically induced bubble knife. The air-liquid interfaces of the dispensed bubbles have novel characteristics which can carry the various kinds of the materials in different phase and viscosity of solution. This technique has advantages in high efficiency of injection of limited amount of valuable samples such as designed m-RNA. In this paper, we demonstrated m-RNA encoding 3xFLAG-GST into xenopus oocyte to be sure that electrically induced bubble knife has ability to injection chemically unstable and biocompatibility. Furthermore, we injected fluorescent beads whose size was 2.1 μm into BY-2 cell as multiphase injection.
AB - We have successfully carried out gene transfer using electrically induced bubble knife. The air-liquid interfaces of the dispensed bubbles have novel characteristics which can carry the various kinds of the materials in different phase and viscosity of solution. This technique has advantages in high efficiency of injection of limited amount of valuable samples such as designed m-RNA. In this paper, we demonstrated m-RNA encoding 3xFLAG-GST into xenopus oocyte to be sure that electrically induced bubble knife has ability to injection chemically unstable and biocompatibility. Furthermore, we injected fluorescent beads whose size was 2.1 μm into BY-2 cell as multiphase injection.
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M3 - Conference contribution
AN - SCOPUS:84907321165
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 1173
EP - 1175
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -