Multiple amino acid substitutions in hemagglutinin are necessary for wild-type measles virus to acquire the ability to use receptor CD46 efficiently

Maino Tahara, Makoto Takeda, Fumio Seki, Takao Hashiguchi, Yusuke Yanagi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Measles virus (MV) possesses two envelope glycoproteins, namely, the receptor-binding hemagglutinin (H) and fusion proteins. Wild-type MV strains isolated in B-lymphoid cell lines use signaling lymphocyte activation molecule (SLAM), but not CD46, as a cellular receptor, whereas MV vaccine strains of the Edmonston lineage use both SLAM and CD46 as receptors. Studies have shown that the residue at position 481 of the H protein is critical in determining the use of CD46 as a receptor. However, the wild-type IC-B strain with a single N481Y substitution in the H protein utilizes CD46 rather inefficiently. In this study, a number of chimeric and mutant H proteins, and recombinant viruses harboring them, were generated to determine which residues of the Edmonston H protein are responsible for its efficient use of CD46. Our results show that three substitutions (N390I and E492G plus N416D or T446S), in addition to N481Y, are necessary for the IC-B H protein to use CD46 efficiently as a receptor. The N3901, N416D, and T446S substitutions are present in the H proteins of all strains of the Edmonston lineage, whereas the E492G substitution is found only in the H protein of the Edmonston tag strain generated from cDNAs. The T484N substitution, found in some of the Edmonston-lineage strains, resulted in a similar effect on the use of CD46 to that caused by the E492G substitution. Thus, multiple residues in the H protein that have not previously been implicated have important roles in the interaction with CD46.

Original languageEnglish
Pages (from-to)2564-2572
Number of pages9
JournalJournal of virology
Volume81
Issue number6
DOIs
Publication statusPublished - Mar 1 2007

Fingerprint

Measles virus
Hemagglutinins
amino acid substitution
hemagglutinins
Amino Acid Substitution
receptors
Proteins
proteins
Lymphocyte Activation
lymphocyte proliferation
Mutant Chimeric Proteins
Measles Vaccine
Glycoproteins
Complementary DNA
glycoproteins
Lymphocytes
Viruses
cell lines
Cell Line
vaccines

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Multiple amino acid substitutions in hemagglutinin are necessary for wild-type measles virus to acquire the ability to use receptor CD46 efficiently. / Tahara, Maino; Takeda, Makoto; Seki, Fumio; Hashiguchi, Takao; Yanagi, Yusuke.

In: Journal of virology, Vol. 81, No. 6, 01.03.2007, p. 2564-2572.

Research output: Contribution to journalArticle

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