Multiple classes of transcription factors regulate the expression of VASCULAR-RELATED NAC-DOMAIN7, a master switch of xylem vessel differentiation

Hitoshi Endo, Masatoshi Yamaguchi, Taizo Tamura, Yoshimi Nakano, Nobuyuki Nishikubo, Arata Yoneda, Ko Kato, Minoru Kubo, Shinya Kajita, Yoshihiro Katayama, Misato Ohtani, Taku Demura

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

The secondary cell walls of xylem cells, including vessel elements, provide mechanical strength and contribute to the conduction of water and minerals. VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a NAC-domain transcription factor that regulates the expression of genes required for xylem vessel element formation. Transient expression assays using 68 transcription factors that are expressed during xylem vessel differentiation showed that 14 transcription factors, including VND1-VND7, are putative positive regulators of VND7 expression. Electrophoretic mobility shift assays revealed that all seven VND proteins bound to the VND7 promoter region at its SMBE/TERE motif, indicating that VND7 is a direct target of all of the VND transcription factors. Overexpression of VND1-VND5, GATA12 and ANAC075, newly identified transcription factors that function upstream of VND7, resulted in ectopic xylem vessel element formation. These data suggest that VND7 transcription is a regulatory target of multiple classes of transcription factors.

Original languageEnglish
Pages (from-to)242-254
Number of pages13
JournalPlant and Cell Physiology
Volume56
Issue number2
DOIs
Publication statusPublished - Feb 1 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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