TY - JOUR
T1 - Multiple classes of transcription factors regulate the expression of VASCULAR-RELATED NAC-DOMAIN7, a master switch of xylem vessel differentiation
AU - Endo, Hitoshi
AU - Yamaguchi, Masatoshi
AU - Tamura, Taizo
AU - Nakano, Yoshimi
AU - Nishikubo, Nobuyuki
AU - Yoneda, Arata
AU - Kato, Ko
AU - Kubo, Minoru
AU - Kajita, Shinya
AU - Katayama, Yoshihiro
AU - Ohtani, Misato
AU - Demura, Taku
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science [KAKENHI grant No. 25840098 to M.Y., 25291062 to T.D.]; the Ministry of Education, Culture, Sports, Science and Technology of Japan [Grant-in-Aid for Scientific Research on Innovative Areas ‘The Plant Cell Wall as Information Processing System’ grant No. 24114002 to T.D., 25114520 to M.O., Grants-in-Aid from the NC-CARP project to T.D.].
Publisher Copyright:
© The Author 2014. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - The secondary cell walls of xylem cells, including vessel elements, provide mechanical strength and contribute to the conduction of water and minerals. VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a NAC-domain transcription factor that regulates the expression of genes required for xylem vessel element formation. Transient expression assays using 68 transcription factors that are expressed during xylem vessel differentiation showed that 14 transcription factors, including VND1-VND7, are putative positive regulators of VND7 expression. Electrophoretic mobility shift assays revealed that all seven VND proteins bound to the VND7 promoter region at its SMBE/TERE motif, indicating that VND7 is a direct target of all of the VND transcription factors. Overexpression of VND1-VND5, GATA12 and ANAC075, newly identified transcription factors that function upstream of VND7, resulted in ectopic xylem vessel element formation. These data suggest that VND7 transcription is a regulatory target of multiple classes of transcription factors.
AB - The secondary cell walls of xylem cells, including vessel elements, provide mechanical strength and contribute to the conduction of water and minerals. VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a NAC-domain transcription factor that regulates the expression of genes required for xylem vessel element formation. Transient expression assays using 68 transcription factors that are expressed during xylem vessel differentiation showed that 14 transcription factors, including VND1-VND7, are putative positive regulators of VND7 expression. Electrophoretic mobility shift assays revealed that all seven VND proteins bound to the VND7 promoter region at its SMBE/TERE motif, indicating that VND7 is a direct target of all of the VND transcription factors. Overexpression of VND1-VND5, GATA12 and ANAC075, newly identified transcription factors that function upstream of VND7, resulted in ectopic xylem vessel element formation. These data suggest that VND7 transcription is a regulatory target of multiple classes of transcription factors.
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U2 - 10.1093/pcp/pcu134
DO - 10.1093/pcp/pcu134
M3 - Article
C2 - 25265867
AN - SCOPUS:84928711578
VL - 56
SP - 242
EP - 254
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 2
ER -