Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe

Tomoko Iwaki, Haruyuki Iefuji, Yoshikazu Hiraga, Akira Hosomi, Tomotake Morita, Yuko Giga-Hama, Kaoru Takegawa

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Sterols are a major class of membrane lipids in eukaryotes. In Schizosaccharomyces pombe, sterol 24-C-methyltransferase (Erg6p), C-8 sterol isomerase (Erg2p), C-5 sterol desaturase (Erg31p, Erg32p), C-22 sterol desaturase (Erg5p) and C-24 (28) sterol reductase (Sts1p/Erg4p) have been predicted, but not yet determined, to catalyse a sequence of reactions from zymosterol to ergosterol. Disruption mutants of these genes were unable to synthesize ergosterol, and most were tolerant to the polyene drugs amphotericin B and nystatin. Disruption of erg31+ or erg32+ did not cause ergosterol deficiency or tolerance to polyene drugs, indicating that the two C-5 sterol desaturases have overlapping functions. GFP-tagged DRM (detergent-resistant membrane)-associated protein Pma1p localized to the plasma membrane in ergΔ mutants. DRM fractionation revealed that the association between Pma1-GFP and DRM was weakened in erg6Δ but not in other erg mutants. Several GFP-tagged plasma membrane proteins were tested, and an amino acid permease homologue, SPBC359.03c, was found to mislocalize to intracellular punctate structures in the ergA mutants. These results indicate that these proteins are responsible for ergosterol biosynthesis in fission yeast, similar to the situation in Saccharomyces cerevisiae. Furthermore, in fission yeast, ergosterol is important for plasma membrane structure and function and for localization of plasma membrane proteins.

Original languageEnglish
Pages (from-to)830-841
Number of pages12
JournalMicrobiology
Volume154
Issue number3
DOIs
Publication statusPublished - Mar 1 2008
Externally publishedYes

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Ergosterol
Schizosaccharomyces
Sterols
Cell Membrane
Detergents
Polyenes
Membrane Proteins
Blood Proteins
Amino Acid Transport Systems
Nystatin
Membranes
Methyltransferases
Amphotericin B
Membrane Lipids
Eukaryota
Pharmaceutical Preparations
Saccharomyces cerevisiae
Oxidoreductases
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Iwaki, T., Iefuji, H., Hiraga, Y., Hosomi, A., Morita, T., Giga-Hama, Y., & Takegawa, K. (2008). Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe. Microbiology, 154(3), 830-841. https://doi.org/10.1099/mic.0.2007/011155-0

Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe. / Iwaki, Tomoko; Iefuji, Haruyuki; Hiraga, Yoshikazu; Hosomi, Akira; Morita, Tomotake; Giga-Hama, Yuko; Takegawa, Kaoru.

In: Microbiology, Vol. 154, No. 3, 01.03.2008, p. 830-841.

Research output: Contribution to journalArticle

Iwaki, T, Iefuji, H, Hiraga, Y, Hosomi, A, Morita, T, Giga-Hama, Y & Takegawa, K 2008, 'Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe', Microbiology, vol. 154, no. 3, pp. 830-841. https://doi.org/10.1099/mic.0.2007/011155-0
Iwaki T, Iefuji H, Hiraga Y, Hosomi A, Morita T, Giga-Hama Y et al. Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe. Microbiology. 2008 Mar 1;154(3):830-841. https://doi.org/10.1099/mic.0.2007/011155-0
Iwaki, Tomoko ; Iefuji, Haruyuki ; Hiraga, Yoshikazu ; Hosomi, Akira ; Morita, Tomotake ; Giga-Hama, Yuko ; Takegawa, Kaoru. / Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe. In: Microbiology. 2008 ; Vol. 154, No. 3. pp. 830-841.
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