Multiple gene duplication and expression of mouse bcl-2-related genes, A1

Shigetsugu Hatakeyama, Azumi Hamasaki, Izumi Negishi, Dennis Y. Loh, Fujiro Sendo, Keiko Nakayama, Kei Ichi Nakayama

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Abstract

Here we report the genomic cloning and characterization of the murine A1 genes, which belong to the bcl-2 gene family. Southern analysis indicated the existence of at least four A1 genes in the murine genome and four different A1 genes, designated A1-a, -b, -c and -d, were cloned from the murine genomic library. The A1-a, -b and -d genes consisted of two exons, whereas the A1-c gene contained 1 bp insertion in the coding region which may result in an aberrant and truncated protein by frame-shift. With the exception of A1-c, the coding regions among A1 genes are highly conserved at > 97% at the nucleotide level and at > 96% at the amino acid level. A1-a, -b and -d genes appeared to be expressed specifically in organs containing many neutrophils. In neutrophils, A1-a, -b and -d transcripts were detected at a comparable level. Our data suggest that the multiple A1 genes in mice were generated by gene duplication and each of them may function as antiapoptotic molecules in neutrophils.

Original languageEnglish
Pages (from-to)631-637
Number of pages7
JournalInternational Immunology
Volume10
Issue number5
DOIs
Publication statusPublished - May 26 1998

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bcl-2 Genes
Gene Duplication
Gene Expression
Genes
Neutrophils
Genomic Library
Organism Cloning
Exons
Nucleotides
Genome
Amino Acids

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Hatakeyama, S., Hamasaki, A., Negishi, I., Loh, D. Y., Sendo, F., Nakayama, K., & Nakayama, K. I. (1998). Multiple gene duplication and expression of mouse bcl-2-related genes, A1. International Immunology, 10(5), 631-637. https://doi.org/10.1093/intimm/10.5.631

Multiple gene duplication and expression of mouse bcl-2-related genes, A1. / Hatakeyama, Shigetsugu; Hamasaki, Azumi; Negishi, Izumi; Loh, Dennis Y.; Sendo, Fujiro; Nakayama, Keiko; Nakayama, Kei Ichi.

In: International Immunology, Vol. 10, No. 5, 26.05.1998, p. 631-637.

Research output: Contribution to journalArticle

Hatakeyama, S, Hamasaki, A, Negishi, I, Loh, DY, Sendo, F, Nakayama, K & Nakayama, KI 1998, 'Multiple gene duplication and expression of mouse bcl-2-related genes, A1', International Immunology, vol. 10, no. 5, pp. 631-637. https://doi.org/10.1093/intimm/10.5.631
Hatakeyama S, Hamasaki A, Negishi I, Loh DY, Sendo F, Nakayama K et al. Multiple gene duplication and expression of mouse bcl-2-related genes, A1. International Immunology. 1998 May 26;10(5):631-637. https://doi.org/10.1093/intimm/10.5.631
Hatakeyama, Shigetsugu ; Hamasaki, Azumi ; Negishi, Izumi ; Loh, Dennis Y. ; Sendo, Fujiro ; Nakayama, Keiko ; Nakayama, Kei Ichi. / Multiple gene duplication and expression of mouse bcl-2-related genes, A1. In: International Immunology. 1998 ; Vol. 10, No. 5. pp. 631-637.
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