Abstract
Detection of the loss of chromosomal regions in cancerous tissues has diagnostic and prognostic relevance, and the development of a reliable and cost-effective technique for this is clinically important. Here we present an efficient technique for quantitative detection of microsatellite alleles, using a post-PCR fluorescence-labeling procedure and multiplexed analysis. We also present a new statistical method for the interpretation of the data that permits reliable and sensitive evaluation of the allelic status of sampled DNA. A high-resolution analysis of allelic imbalance on chromosomes 1p, 10 and 19q in 28 glioma samples of various types using this method revealed that allelic imbalances are more frequent than have been reported, suggesting the diagnostic value of this method in examining the genetic profiles of gliomas.
| Original language | English |
|---|---|
| Pages (from-to) | 284-290 |
| Number of pages | 7 |
| Journal | Japanese Journal of Cancer Research |
| Volume | 93 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 2002 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
Cite this
Multiplexed analysis of post-PCR fluorescence-labeled microsatellite alleles and statistical evaluation of their imbalance in brain tumors. / Yoshimoto, Koji; Iwaki, Toru; Inamura, Takanori; Fukui, Masashi; Tahira, Tomoko; Hayashi, Kenshi.
In: Japanese Journal of Cancer Research, Vol. 93, No. 3, 01.01.2002, p. 284-290.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multiplexed analysis of post-PCR fluorescence-labeled microsatellite alleles and statistical evaluation of their imbalance in brain tumors
AU - Yoshimoto, Koji
AU - Iwaki, Toru
AU - Inamura, Takanori
AU - Fukui, Masashi
AU - Tahira, Tomoko
AU - Hayashi, Kenshi
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Detection of the loss of chromosomal regions in cancerous tissues has diagnostic and prognostic relevance, and the development of a reliable and cost-effective technique for this is clinically important. Here we present an efficient technique for quantitative detection of microsatellite alleles, using a post-PCR fluorescence-labeling procedure and multiplexed analysis. We also present a new statistical method for the interpretation of the data that permits reliable and sensitive evaluation of the allelic status of sampled DNA. A high-resolution analysis of allelic imbalance on chromosomes 1p, 10 and 19q in 28 glioma samples of various types using this method revealed that allelic imbalances are more frequent than have been reported, suggesting the diagnostic value of this method in examining the genetic profiles of gliomas.
AB - Detection of the loss of chromosomal regions in cancerous tissues has diagnostic and prognostic relevance, and the development of a reliable and cost-effective technique for this is clinically important. Here we present an efficient technique for quantitative detection of microsatellite alleles, using a post-PCR fluorescence-labeling procedure and multiplexed analysis. We also present a new statistical method for the interpretation of the data that permits reliable and sensitive evaluation of the allelic status of sampled DNA. A high-resolution analysis of allelic imbalance on chromosomes 1p, 10 and 19q in 28 glioma samples of various types using this method revealed that allelic imbalances are more frequent than have been reported, suggesting the diagnostic value of this method in examining the genetic profiles of gliomas.
UR - http://www.scopus.com/inward/record.url?scp=0036236410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036236410&partnerID=8YFLogxK
U2 - 10.1111/j.1349-7006.2002.tb02170.x
DO - 10.1111/j.1349-7006.2002.tb02170.x
M3 - Article
C2 - 11927010
AN - SCOPUS:0036236410
VL - 93
SP - 284
EP - 290
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 3
ER -