TY - JOUR
T1 - Muscarinic acetylcholine receptor subtypes in the human iris
AU - Ishizaka, Nobuto
AU - Noda, Mami
AU - Yokoyama, Shigeru
AU - Kawasaki, Kazuo
AU - Yamamoto, Miyuki
AU - Higashida, Haruhiro
PY - 1998/3/23
Y1 - 1998/3/23
N2 - Employing subtype-specific antisera, we measured the relative immunoreactivity of five muscarinic acetylcholine receptor (mAChR) subtype proteins (m1-m5) in the human iris. The most intensive FITC immunofluorescence was detected by the anti-m3 antibody, followed by anti-ml and -m5 antisera, in the iris sphincter muscle cells. Only very weak fluorescence was obtained by anti-m2 and -m4 antibodies. In dilator muscle cells, weak but not consistent immunoreactivity was found by anti-ml and -m5 antibodies. The results suggest that the m3 muscarinic receptor is the predominant subtype in sphincter muscle cells of the human iris.
AB - Employing subtype-specific antisera, we measured the relative immunoreactivity of five muscarinic acetylcholine receptor (mAChR) subtype proteins (m1-m5) in the human iris. The most intensive FITC immunofluorescence was detected by the anti-m3 antibody, followed by anti-ml and -m5 antisera, in the iris sphincter muscle cells. Only very weak fluorescence was obtained by anti-m2 and -m4 antibodies. In dilator muscle cells, weak but not consistent immunoreactivity was found by anti-ml and -m5 antibodies. The results suggest that the m3 muscarinic receptor is the predominant subtype in sphincter muscle cells of the human iris.
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U2 - 10.1016/S0006-8993(97)01554-0
DO - 10.1016/S0006-8993(97)01554-0
M3 - Article
C2 - 9518684
AN - SCOPUS:0032559882
VL - 787
SP - 344
EP - 347
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -