Muscarinic stimulation of calcium influx and norepinephrine release in PC12 cells

Kazuhide Inoue, J. G. Kenimer

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Muscarinic cholinergic receptor stimulation evokes catecholamine secretion from some cell types, but the mechanism has not been well characterized. Using pheochromocytoma (PC12) cells, we show that the muscarinic agonist methacholine stimulates 45Ca2+ influx and [3H]norepinephrine release in a dose-dependent manner. Experiments performed in Na+-free medium or with inhibitors of voltage-dependent Ca2+ channels suggest the involvement of a receptor-activated Ca2+ channel which differs significantly from the voltage-dependent Ca2+ channel involved in nicotinic receptor-stimulated release. Furthermore, both influx and release were inhibited by pertussis toxin (0.5-2.0 ng/ml, 21 h) with a dose dependency which paralleled the dose dependency of pertussis toxin-dependent in vivo ADP-ribosylation of a 41-kDa protein. These experiments provide the first evidence that muscarinic stimulation evokes neurotransmitter secretion by opening a receptor-activated Ca2+ channel which is controlled by a pertussis toxin-sensitive protein.

Original languageEnglish
Pages (from-to)8157-8161
Number of pages5
JournalJournal of Biological Chemistry
Volume263
Issue number17
Publication statusPublished - Jan 1 1988

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PC12 Cells
Pertussis Toxin
Cholinergic Agents
Norepinephrine
Calcium
Muscarinic Agonists
Methacholine Chloride
Nicotinic Receptors
Electric potential
Pheochromocytoma
Cholinergic Receptors
Muscarinic Receptors
Adenosine Diphosphate
Catecholamines
Neurotransmitter Agents
Proteins
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Muscarinic stimulation of calcium influx and norepinephrine release in PC12 cells. / Inoue, Kazuhide; Kenimer, J. G.

In: Journal of Biological Chemistry, Vol. 263, No. 17, 01.01.1988, p. 8157-8161.

Research output: Contribution to journalArticle

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