Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes

Zhao Chong, Haruka Matsuo, Mai Kuroda, Shuntaro Yamashita, Gopal Prasad Parajuli, Hira Kaji Manandhar, Kuniyoshi Shimizu, Yoshinori Katakura

Research output: Contribution to journalArticle

Abstract

Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.

Original languageEnglish
Pages (from-to)1001-1008
Number of pages8
JournalCytotechnology
Volume70
Issue number3
DOIs
Publication statusPublished - Jun 1 2018

Fingerprint

Cell Aging
Agaricales
Keratinocytes
Galactosidases
Irradiation
Bioactivity
Screening
Trametes
Nepal
Cytosol
Food

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Chong, Z., Matsuo, H., Kuroda, M., Yamashita, S., Parajuli, G. P., Manandhar, H. K., ... Katakura, Y. (2018). Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes. Cytotechnology, 70(3), 1001-1008. https://doi.org/10.1007/s10616-018-0229-1

Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes. / Chong, Zhao; Matsuo, Haruka; Kuroda, Mai; Yamashita, Shuntaro; Parajuli, Gopal Prasad; Manandhar, Hira Kaji; Shimizu, Kuniyoshi; Katakura, Yoshinori.

In: Cytotechnology, Vol. 70, No. 3, 01.06.2018, p. 1001-1008.

Research output: Contribution to journalArticle

Chong, Z, Matsuo, H, Kuroda, M, Yamashita, S, Parajuli, GP, Manandhar, HK, Shimizu, K & Katakura, Y 2018, 'Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes', Cytotechnology, vol. 70, no. 3, pp. 1001-1008. https://doi.org/10.1007/s10616-018-0229-1
Chong Z, Matsuo H, Kuroda M, Yamashita S, Parajuli GP, Manandhar HK et al. Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes. Cytotechnology. 2018 Jun 1;70(3):1001-1008. https://doi.org/10.1007/s10616-018-0229-1
Chong, Zhao ; Matsuo, Haruka ; Kuroda, Mai ; Yamashita, Shuntaro ; Parajuli, Gopal Prasad ; Manandhar, Hira Kaji ; Shimizu, Kuniyoshi ; Katakura, Yoshinori. / Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes. In: Cytotechnology. 2018 ; Vol. 70, No. 3. pp. 1001-1008.
@article{3827e808564942718703692490e537aa,
title = "Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes",
abstract = "Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.",
author = "Zhao Chong and Haruka Matsuo and Mai Kuroda and Shuntaro Yamashita and Parajuli, {Gopal Prasad} and Manandhar, {Hira Kaji} and Kuniyoshi Shimizu and Yoshinori Katakura",
year = "2018",
month = "6",
day = "1",
doi = "10.1007/s10616-018-0229-1",
language = "English",
volume = "70",
pages = "1001--1008",
journal = "Cytotechnology",
issn = "0920-9069",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes

AU - Chong, Zhao

AU - Matsuo, Haruka

AU - Kuroda, Mai

AU - Yamashita, Shuntaro

AU - Parajuli, Gopal Prasad

AU - Manandhar, Hira Kaji

AU - Shimizu, Kuniyoshi

AU - Katakura, Yoshinori

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.

AB - Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.

UR - http://www.scopus.com/inward/record.url?scp=85047914454&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047914454&partnerID=8YFLogxK

U2 - 10.1007/s10616-018-0229-1

DO - 10.1007/s10616-018-0229-1

M3 - Article

AN - SCOPUS:85047914454

VL - 70

SP - 1001

EP - 1008

JO - Cytotechnology

JF - Cytotechnology

SN - 0920-9069

IS - 3

ER -