Mutants disrupted in the type iii secretion system of bradyrhizobium elkanii bly3-8 overcame nodulation restriction by rj3-genotype soybean

Miku Shobudani; Aung Zaw Htwe; Takeo Yamakawa; Matsujiro Ishibashi; Hirohito Tsurumaru

doi:10.1264/jsme2.ME19151

Mutants disrupted in the type iii secretion system of bradyrhizobium elkanii bly3-8 overcame nodulation restriction by rj₃-genotype soybean

Miku Shobudani, Aung Zaw Htwe, Takeo Yamakawa, Matsujiro Ishibashi, Hirohito Tsurumaru

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Bradyrhizobium elkanii BLY3-8 does not form nodules on the roots of Rj₃-genotype soybean (cultivar D-51). This is a cultivar-specific nodulation restriction. The genes A6X20_40975 and A6X20_41030 in strain BLY3-8 were predicted to encode the transcriptional activator and apparatus of the type III secretion system (T3SS) (the proteins TtsI and RhcJ), respectively. Mutants disrupted in these genes overcame the nodulation restriction. These results suggest that an effector injected via T3SS into Rj₃-genotype soybean is involved in nodulation restriction by Rj₃-genotype soybean.

Original language	English
Article number	ME19151
Journal	Microbes and environments
Volume	35
Issue number	2
DOIs	https://doi.org/10.1264/jsme2.ME19151
Publication status	Published - 2020

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

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title = "Mutants disrupted in the type iii secretion system of bradyrhizobium elkanii bly3-8 overcame nodulation restriction by rj3-genotype soybean",

abstract = "Bradyrhizobium elkanii BLY3-8 does not form nodules on the roots of Rj3-genotype soybean (cultivar D-51). This is a cultivar-specific nodulation restriction. The genes A6X20_40975 and A6X20_41030 in strain BLY3-8 were predicted to encode the transcriptional activator and apparatus of the type III secretion system (T3SS) (the proteins TtsI and RhcJ), respectively. Mutants disrupted in these genes overcame the nodulation restriction. These results suggest that an effector injected via T3SS into Rj3-genotype soybean is involved in nodulation restriction by Rj3-genotype soybean.",

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journal = "Microbes and Environments",

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T1 - Mutants disrupted in the type iii secretion system of bradyrhizobium elkanii bly3-8 overcame nodulation restriction by rj3-genotype soybean

AU - Shobudani, Miku

AU - Htwe, Aung Zaw

AU - Yamakawa, Takeo

AU - Ishibashi, Matsujiro

AU - Tsurumaru, Hirohito

PY - 2020

Y1 - 2020

N2 - Bradyrhizobium elkanii BLY3-8 does not form nodules on the roots of Rj3-genotype soybean (cultivar D-51). This is a cultivar-specific nodulation restriction. The genes A6X20_40975 and A6X20_41030 in strain BLY3-8 were predicted to encode the transcriptional activator and apparatus of the type III secretion system (T3SS) (the proteins TtsI and RhcJ), respectively. Mutants disrupted in these genes overcame the nodulation restriction. These results suggest that an effector injected via T3SS into Rj3-genotype soybean is involved in nodulation restriction by Rj3-genotype soybean.

AB - Bradyrhizobium elkanii BLY3-8 does not form nodules on the roots of Rj3-genotype soybean (cultivar D-51). This is a cultivar-specific nodulation restriction. The genes A6X20_40975 and A6X20_41030 in strain BLY3-8 were predicted to encode the transcriptional activator and apparatus of the type III secretion system (T3SS) (the proteins TtsI and RhcJ), respectively. Mutants disrupted in these genes overcame the nodulation restriction. These results suggest that an effector injected via T3SS into Rj3-genotype soybean is involved in nodulation restriction by Rj3-genotype soybean.

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Mutants disrupted in the type iii secretion system of bradyrhizobium elkanii bly3-8 overcame nodulation restriction by rj₃-genotype soybean

Abstract

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