Mutation analysis utilizing DNA intercalation in reversed micelles

Park Lianchun, Miki Funai, Masahiro Goto

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A new mutation analysis system was developed by utilizing a DNA intercalator in reversed micelles. The hybridization signal was amplified by monitoring the intercalation behavior between a porphirine derivative and a double strand DNA in reversed micelles. Hybridization rate in reversed micelles was analyzed for three sample DNAs bearing a mutation in a different part. The location of the mutation significantly affected the hybridization behavior. This novel method allows the DNA concentration to be reduced to less than one-tenth of that employed in the conventional reversed micellar method.

    Original languageEnglish
    Pages (from-to)776-778
    Number of pages3
    Journalkagaku kogaku ronbunshu
    Volume28
    Issue number6
    DOIs
    Publication statusPublished - Nov 2002

    All Science Journal Classification (ASJC) codes

    • Chemistry(all)
    • Chemical Engineering(all)

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