Mutation analysis utilizing DNA intercalation in reversed micelles

Park Lianchun, Miki Funai, Masahiro Goto

Research output: Contribution to journalArticlepeer-review

Abstract

A new mutation analysis system was developed by utilizing a DNA intercalator in reversed micelles. The hybridization signal was amplified by monitoring the intercalation behavior between a porphirine derivative and a double strand DNA in reversed micelles. Hybridization rate in reversed micelles was analyzed for three sample DNAs bearing a mutation in a different part. The location of the mutation significantly affected the hybridization behavior. This novel method allows the DNA concentration to be reduced to less than one-tenth of that employed in the conventional reversed micellar method.

Original languageEnglish
Pages (from-to)776-778
Number of pages3
JournalKagaku Kogaku Ronbunshu
Volume28
Issue number6
DOIs
Publication statusPublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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