Mutation analysis utilizing DNA intercalation in reversed micelles

Park Lianchun, Miki Funai, Masahiro Goto

Research output: Contribution to journalArticle

Abstract

A new mutation analysis system was developed by utilizing a DNA intercalator in reversed micelles. The hybridization signal was amplified by monitoring the intercalation behavior between a porphirine derivative and a double strand DNA in reversed micelles. Hybridization rate in reversed micelles was analyzed for three sample DNAs bearing a mutation in a different part. The location of the mutation significantly affected the hybridization behavior. This novel method allows the DNA concentration to be reduced to less than one-tenth of that employed in the conventional reversed micellar method.

Original languageEnglish
Pages (from-to)776-778
Number of pages3
JournalKagaku Kogaku Ronbunshu
Volume28
Issue number6
DOIs
Publication statusPublished - Jan 1 2002

Fingerprint

Micelles
Intercalation
DNA
Bearings (structural)
Intercalating Agents
Derivatives
Monitoring

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Mutation analysis utilizing DNA intercalation in reversed micelles. / Lianchun, Park; Funai, Miki; Goto, Masahiro.

In: Kagaku Kogaku Ronbunshu, Vol. 28, No. 6, 01.01.2002, p. 776-778.

Research output: Contribution to journalArticle

Lianchun, Park ; Funai, Miki ; Goto, Masahiro. / Mutation analysis utilizing DNA intercalation in reversed micelles. In: Kagaku Kogaku Ronbunshu. 2002 ; Vol. 28, No. 6. pp. 776-778.
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