Mutation detection in the drug-resistant hepatitis B virus polymerase gene using nanostructured reverse micelles

Lian Chun Park, Tatsuo Maruyama, Noriho Kamiya, Masahiro Goto, Hiroyuki Kuma, Naotaka Hamasaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The emergence of drug-resistant hepatitis B virus (HBV) has been reported in patients with prolonged administration of lamivudine, which is a potent drug for the prevention of HBV infection. Lamivudine-resistant HBV has several types of mutations at the YMDD motif of its DNA polymerase. We successfully demonstrated that monitoring the hybridization behavior in nanostructured reverse micelles enables us to detect single nucleotide polymorphisms (SNPs). With the aid of reverse micelles, a model 40-mer oligonucleotide containing a single-base substitution was clearly distinguished from the normal, complementary oligonucleotide. In addition, we extended this technique to a high-throughput analysis. The results obtained with a 96-well micro-plate reader indicated the possibility of SNPs detection toward multiple samples of patients.

Original languageEnglish
Pages (from-to)1609-1611
Number of pages3
Journalanalytical sciences
Volume20
Issue number11
DOIs
Publication statusPublished - Nov 2004

Fingerprint

Micelles
Viruses
Lamivudine
Genes
Polymorphism
Oligonucleotides
Nucleotides
Pharmaceutical Preparations
DNA-Directed DNA Polymerase
Substitution reactions
Throughput
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Mutation detection in the drug-resistant hepatitis B virus polymerase gene using nanostructured reverse micelles. / Park, Lian Chun; Maruyama, Tatsuo; Kamiya, Noriho; Goto, Masahiro; Kuma, Hiroyuki; Hamasaki, Naotaka.

In: analytical sciences, Vol. 20, No. 11, 11.2004, p. 1609-1611.

Research output: Contribution to journalArticle

Park, Lian Chun ; Maruyama, Tatsuo ; Kamiya, Noriho ; Goto, Masahiro ; Kuma, Hiroyuki ; Hamasaki, Naotaka. / Mutation detection in the drug-resistant hepatitis B virus polymerase gene using nanostructured reverse micelles. In: analytical sciences. 2004 ; Vol. 20, No. 11. pp. 1609-1611.
@article{5ad51d8922444947967b626a17ba2877,
title = "Mutation detection in the drug-resistant hepatitis B virus polymerase gene using nanostructured reverse micelles",
abstract = "The emergence of drug-resistant hepatitis B virus (HBV) has been reported in patients with prolonged administration of lamivudine, which is a potent drug for the prevention of HBV infection. Lamivudine-resistant HBV has several types of mutations at the YMDD motif of its DNA polymerase. We successfully demonstrated that monitoring the hybridization behavior in nanostructured reverse micelles enables us to detect single nucleotide polymorphisms (SNPs). With the aid of reverse micelles, a model 40-mer oligonucleotide containing a single-base substitution was clearly distinguished from the normal, complementary oligonucleotide. In addition, we extended this technique to a high-throughput analysis. The results obtained with a 96-well micro-plate reader indicated the possibility of SNPs detection toward multiple samples of patients.",
author = "Park, {Lian Chun} and Tatsuo Maruyama and Noriho Kamiya and Masahiro Goto and Hiroyuki Kuma and Naotaka Hamasaki",
year = "2004",
month = "11",
doi = "10.2116/analsci.20.1609",
language = "English",
volume = "20",
pages = "1609--1611",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "The Japan Society for Analytical Chemistry",
number = "11",

}

TY - JOUR

T1 - Mutation detection in the drug-resistant hepatitis B virus polymerase gene using nanostructured reverse micelles

AU - Park, Lian Chun

AU - Maruyama, Tatsuo

AU - Kamiya, Noriho

AU - Goto, Masahiro

AU - Kuma, Hiroyuki

AU - Hamasaki, Naotaka

PY - 2004/11

Y1 - 2004/11

N2 - The emergence of drug-resistant hepatitis B virus (HBV) has been reported in patients with prolonged administration of lamivudine, which is a potent drug for the prevention of HBV infection. Lamivudine-resistant HBV has several types of mutations at the YMDD motif of its DNA polymerase. We successfully demonstrated that monitoring the hybridization behavior in nanostructured reverse micelles enables us to detect single nucleotide polymorphisms (SNPs). With the aid of reverse micelles, a model 40-mer oligonucleotide containing a single-base substitution was clearly distinguished from the normal, complementary oligonucleotide. In addition, we extended this technique to a high-throughput analysis. The results obtained with a 96-well micro-plate reader indicated the possibility of SNPs detection toward multiple samples of patients.

AB - The emergence of drug-resistant hepatitis B virus (HBV) has been reported in patients with prolonged administration of lamivudine, which is a potent drug for the prevention of HBV infection. Lamivudine-resistant HBV has several types of mutations at the YMDD motif of its DNA polymerase. We successfully demonstrated that monitoring the hybridization behavior in nanostructured reverse micelles enables us to detect single nucleotide polymorphisms (SNPs). With the aid of reverse micelles, a model 40-mer oligonucleotide containing a single-base substitution was clearly distinguished from the normal, complementary oligonucleotide. In addition, we extended this technique to a high-throughput analysis. The results obtained with a 96-well micro-plate reader indicated the possibility of SNPs detection toward multiple samples of patients.

UR - http://www.scopus.com/inward/record.url?scp=10444254615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10444254615&partnerID=8YFLogxK

U2 - 10.2116/analsci.20.1609

DO - 10.2116/analsci.20.1609

M3 - Article

C2 - 15566158

AN - SCOPUS:10444254615

VL - 20

SP - 1609

EP - 1611

JO - Analytical Sciences

JF - Analytical Sciences

SN - 0910-6340

IS - 11

ER -