Mutation detection in the drug-resistant hepatitis B virus polymerase gene using nanostructured reverse micelles

Lian Chun Park, Tatsuo Maruyama, Noriho Kamiya, Masahiro Goto, Hiroyuki Kuma, Naotaka Hamasaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The emergence of drug-resistant hepatitis B virus (HBV) has been reported in patients with prolonged administration of lamivudine, which is a potent drug for the prevention of HBV infection. Lamivudine-resistant HBV has several types of mutations at the YMDD motif of its DNA polymerase. We successfully demonstrated that monitoring the hybridization behavior in nanostructured reverse micelles enables us to detect single nucleotide polymorphisms (SNPs). With the aid of reverse micelles, a model 40-mer oligonucleotide containing a single-base substitution was clearly distinguished from the normal, complementary oligonucleotide. In addition, we extended this technique to a high-throughput analysis. The results obtained with a 96-well micro-plate reader indicated the possibility of SNPs detection toward multiple samples of patients.

Original languageEnglish
Pages (from-to)1609-1611
Number of pages3
Journalanalytical sciences
Volume20
Issue number11
DOIs
Publication statusPublished - Nov 2004

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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