Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1

Asami Sugihara, Luan Cao Nguyen, Hossain Mohammad Shamim, Tetsushi Iida, Mai Nakase, Kaoru Takegawa, Mitsuhisa Senda, Shohei Jida, Masaru Ueno

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fission yeast Pik1p is one of three phosphatidylinositol 4-kinases associated with the Golgi complex, but its function is not fully understood. Deletion of pot1+ causes telomere degradation and chromosome circularization. We searched for the gene which becomes synthetically lethal with pot1Δ. We obtained a novel pik1 mutant, pik1-1, which is synthetically lethal with pot1Δ. We found phosphoinositol 4-phosphate in the Golgi was reduced in pik1-1. To investigate the mechanism of the lethality of the pot1Δ pik1-1 double mutant, we constructed the nmt-pot1-aid pik1-1 strain, where Pot1 function becomes low by drugs, which leads to telomere loss and chromosome circularization, and found pik1-1 mutation does not affect telomere resection and chromosome circularization. Thus, our results suggest that pik1+ is required for the maintenance of circular chromosomes.

Original languageEnglish
Pages (from-to)1284-1290
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume496
Issue number4
DOIs
Publication statusPublished - Feb 19 2018

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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