Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, bombyx mori

Lingyan Wang, Takashi Kiuchi, Tsuguru Fujii, Takaaki Daimon, Muwang Li, Yutaka Banno, Shingo Kikuta, Takahiro Kikawada, Susumu Katsuma, Toru Shimada

Research output: Contribution to journalArticle

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Abstract

ok mutants of the silkworm, Bombyx mori, exhibit highly translucent larval skin resulting from the inability to incorporate uric acid into the epidermal cells. Here we report the identification of a gene responsible for the ok mutation using positional cloning and RNAi experiments. In two independent ok mutant strains, we found a 49-bp deletion and a 233-bp duplication, respectively, in mRNAs of a novel gene, Bm-. ok, which encodes a half-type ABC transporter, each of which results in translation of a truncated protein in each mutant. Although the Bm-. ok sequence was homologous to well-known transporter genes, white, scarlet, and brown in Drosophila, the discovery of novel orthologs in the genomes of lepidopteran, hymenopteran, and hemipteran insects identifies it as a member of a new distinct subfamily of transporters. Embryonic RNAi of Bm-. ok demonstrated that repression of Bm-. ok causes a translucent phenotype in the first-instar silkworm larva. We discuss the possibility that Bm-ok forms a heterodimer with another half-type ABC transporter, Bmwh3, and acts as a uric acid transporter in the silkworm epidermis.

Original languageEnglish
Pages (from-to)562-571
Number of pages10
JournalInsect Biochemistry and Molecular Biology
Volume43
Issue number7
DOIs
Publication statusPublished - Jul 1 2013

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ABC transporters
ATP-Binding Cassette Transporters
Bombyx
uric acid
Bombyx mori
silkworms
Uric Acid
Epidermis
transporters
Genes
mutation
mutants
Mutation
RNA Interference
genes
translation (genetics)
insect larvae
molecular cloning
Cloning
Drosophila

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, bombyx mori. / Wang, Lingyan; Kiuchi, Takashi; Fujii, Tsuguru; Daimon, Takaaki; Li, Muwang; Banno, Yutaka; Kikuta, Shingo; Kikawada, Takahiro; Katsuma, Susumu; Shimada, Toru.

In: Insect Biochemistry and Molecular Biology, Vol. 43, No. 7, 01.07.2013, p. 562-571.

Research output: Contribution to journalArticle

Wang, Lingyan ; Kiuchi, Takashi ; Fujii, Tsuguru ; Daimon, Takaaki ; Li, Muwang ; Banno, Yutaka ; Kikuta, Shingo ; Kikawada, Takahiro ; Katsuma, Susumu ; Shimada, Toru. / Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, bombyx mori. In: Insect Biochemistry and Molecular Biology. 2013 ; Vol. 43, No. 7. pp. 562-571.
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