Mutational analysis of the Pyrococcus furiosus Holliday junction resolvase hjc revealed functionally important residues for dimer formation, junction DNA binding, and cleavage activities

Kayoko Komori, Shinzi Sakae, Hiromi Daiyasu, Hiroyuki Toh, Kosuke Morikawa, Hideo Shinagawa, Yoshizumi Ishino

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The Holliday junction cleavage protein, Hjc resolvase of Pyrococcus furiosus, is the first Holliday junction resolvase to be discovered in Archaea. Although the archaeal resolvase shares certain biochemical properties with other non-archaeal junction resolvases, no amino acid sequence similarity has been identified. To investigate the structure-function relationship of this new Holliday junction resolvase, we constructed a series of mutant hjc genes using site-directed mutagenesis targeted at the residues conserved among the archaeal orthologs. The products of these mutant genes were purified to homogeneity. With analysis of the activity of the mutant proteins to bind and cleave synthetic Holliday junctions, one acidic residue, Glu-9, and two basic residues, Arg-10 and Arg-25, were found to play critical roles in enzyme action. This is in addition to the three conserved residues, Asp-33, Glu-46, and Lys-48, which are also conserved in the motif found in the type II restriction endonuclease family proteins. Two aromatic residues, Phe-68 and Phe-72, are important for the formation of the homodimer probably through hydrophobic interactions. The results of these studies have provided insights into the structure-function relationships of the archaeal Holliday junction resolvase as well as the universality and diversity of the Holliday junction cleavage reaction.

Original languageEnglish
Pages (from-to)40385-40391
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number51
DOIs
Publication statusPublished - Dec 22 2000
Externally publishedYes

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Holliday Junction Resolvases
Pyrococcus furiosus
Cruciform DNA
DNA Cleavage
Recombinases
Dimers
DNA
Type II Site Specific Deoxyribonucleases
Genes
Mutagenesis
Archaea
Mutant Proteins
Site-Directed Mutagenesis
Hydrophobic and Hydrophilic Interactions
Amino Acid Sequence
Proteins
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mutational analysis of the Pyrococcus furiosus Holliday junction resolvase hjc revealed functionally important residues for dimer formation, junction DNA binding, and cleavage activities. / Komori, Kayoko; Sakae, Shinzi; Daiyasu, Hiromi; Toh, Hiroyuki; Morikawa, Kosuke; Shinagawa, Hideo; Ishino, Yoshizumi.

In: Journal of Biological Chemistry, Vol. 275, No. 51, 22.12.2000, p. 40385-40391.

Research output: Contribution to journalArticle

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