Mutations affecting nerve attachment of Caenorhabditis elegans

G. Shioi, M. Shoji, M. Nakamura, Takeshi Ishihara, I. Katsura, H. Fujisawa, S. Takagi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Using a pan-neuronal GFP marker, a morphological screen was performed to detect Caenorhabditis elegans larval lethal mutants with severely disorganized major nerve cords. We recovered and characterized 21 mutants that displayed displacement or detachment of the ventral nerve cord from the body wall (Ven: ventral cord abnormal). Six mutations defined three novel genetic loci: ven-1, ven-2, and ven-3. Fifteen mutations proved to be alleles of previously identified muscle attachment/positioning genes, mup-4, mua-1, mua-5, and mua-6. All the mutants also displayed muscle attachment/positioning defects characteristic of mua/mup mutants. The pan-neuronal GFP marker also revealed that mutants of other mua/mup loci, such as mup-1, mup-2 and mua-2, exhibited the Ven defect. The hypodermis, the excretory canal, and the gonad were morphologically abnormal in some of the mutants. The pleiotropic nature of the defects indicates that ven and mua/mup genes are required generally for the maintenance of attachment of tissues to the body wall in C. elegans.

Original languageEnglish
Pages (from-to)1611-1622
Number of pages12
JournalGenetics
Volume157
Issue number4
Publication statusPublished - Apr 25 2001
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Muscles
Mutation
Genetic Loci
Subcutaneous Tissue
Gonads
Genes
Alleles
Maintenance

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Shioi, G., Shoji, M., Nakamura, M., Ishihara, T., Katsura, I., Fujisawa, H., & Takagi, S. (2001). Mutations affecting nerve attachment of Caenorhabditis elegans. Genetics, 157(4), 1611-1622.

Mutations affecting nerve attachment of Caenorhabditis elegans. / Shioi, G.; Shoji, M.; Nakamura, M.; Ishihara, Takeshi; Katsura, I.; Fujisawa, H.; Takagi, S.

In: Genetics, Vol. 157, No. 4, 25.04.2001, p. 1611-1622.

Research output: Contribution to journalArticle

Shioi, G, Shoji, M, Nakamura, M, Ishihara, T, Katsura, I, Fujisawa, H & Takagi, S 2001, 'Mutations affecting nerve attachment of Caenorhabditis elegans', Genetics, vol. 157, no. 4, pp. 1611-1622.
Shioi G, Shoji M, Nakamura M, Ishihara T, Katsura I, Fujisawa H et al. Mutations affecting nerve attachment of Caenorhabditis elegans. Genetics. 2001 Apr 25;157(4):1611-1622.
Shioi, G. ; Shoji, M. ; Nakamura, M. ; Ishihara, Takeshi ; Katsura, I. ; Fujisawa, H. ; Takagi, S. / Mutations affecting nerve attachment of Caenorhabditis elegans. In: Genetics. 2001 ; Vol. 157, No. 4. pp. 1611-1622.
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