Mutations of the p53 gene in malignant rhabdoid tumors of soft tissue and the kidney: Immunohistochemical and DNA direct sequencing analysis

Yoshiaki Kinoshita, Hideki Shiratsuchi, Sadafumi Tamiya, Yumi Oshiro, Yoichi Hachitanda, Yoshinao Oda, Sachiyo Suita, Masazumi Tsuneyoshi

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Abstract

Malignant rhabdoid tumor (MRT) is characterized by the presence of intracytoplasmic eosinophilic inclusions composed of whorls of intermediate filaments. This tumor was originally described as an entity of the abortive type of Wilms' tumor in childhood. Recently, it has been proved that these rhabdoid cells can be observed in various types of malignant tumors, including soft tissue sarcoma or carcinoma. To investigate the oncogenesis of this tumor, we examined the p53 gene alteration by means of immunohistochemical analysis and DNA direct sequencing in three cases of malignant rhabdoid tumor (MRT) of the soft tissue and three cases of MRT of the kidney. All the cases of MRT of the soft tissue and two of the cases of MRT of the kidney showed immunopositivity for p53 protein. Among them, one of the cases of MRT of the soft tissue and two of the cases of MRT of the kidney showed missense mutations of the p53 gene. These results strongly suggest that p53 gene alterations may have an important role to play in the aggressive biological behavior and poor prognosis of this tumor.

Original languageEnglish
Pages (from-to)351-358
Number of pages8
JournalJournal of Cancer Research and Clinical Oncology
Volume127
Issue number6
DOIs
Publication statusPublished - May 29 2001

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Rhabdoid Tumor
p53 Genes
DNA Sequence Analysis
Kidney
Mutation
Neoplasms
Wilms Tumor
Intermediate Filaments
Missense Mutation
Sarcoma
Carcinogenesis
Carcinoma

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Mutations of the p53 gene in malignant rhabdoid tumors of soft tissue and the kidney : Immunohistochemical and DNA direct sequencing analysis. / Kinoshita, Yoshiaki; Shiratsuchi, Hideki; Tamiya, Sadafumi; Oshiro, Yumi; Hachitanda, Yoichi; Oda, Yoshinao; Suita, Sachiyo; Tsuneyoshi, Masazumi.

In: Journal of Cancer Research and Clinical Oncology, Vol. 127, No. 6, 29.05.2001, p. 351-358.

Research output: Contribution to journalArticle

Kinoshita, Yoshiaki ; Shiratsuchi, Hideki ; Tamiya, Sadafumi ; Oshiro, Yumi ; Hachitanda, Yoichi ; Oda, Yoshinao ; Suita, Sachiyo ; Tsuneyoshi, Masazumi. / Mutations of the p53 gene in malignant rhabdoid tumors of soft tissue and the kidney : Immunohistochemical and DNA direct sequencing analysis. In: Journal of Cancer Research and Clinical Oncology. 2001 ; Vol. 127, No. 6. pp. 351-358.
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