Abstract
Normal silkworms (Bombyx mori) have opaque larval skin due to uric acid accumulation in the epidermis while a mutant, og, is translucent owing to a deficiency in xanthine dehydrogenase (XDH), which synthesizes uric acid. Molybdenum cofactor (MoCo) sulfurase is responsible for XDH activation in various organisms. A silkworm MoCo sulfurase gene was cloned and found to be on the og locus, whose mutant alleles, ogk and ogt, show premature stop codons, proving that og is the MoCo sulfurase gene. It was observed that a miniature inverted-repeat transposable element (MITE), named Organdy, when inserted in an ogt mutant allele exon, causes unstable splicing of a downstream intron leading to incomplete open reading frames.
| Original language | English |
|---|---|
| Pages (from-to) | 417-427 |
| Number of pages | 11 |
| Journal | Insect Biochemistry and Molecular Biology |
| Volume | 33 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1 2003 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Insect Science
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Mutations of the silkworm molybdenum cofactor sulfurase gene, og, cause translucent larval skin. / Kômoto, N.; Sezutsu, H.; Yukuhiro, K.; Banno, Yutaka; Fujii, H.
In: Insect Biochemistry and Molecular Biology, Vol. 33, No. 4, 01.04.2003, p. 417-427.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mutations of the silkworm molybdenum cofactor sulfurase gene, og, cause translucent larval skin
AU - Kômoto, N.
AU - Sezutsu, H.
AU - Yukuhiro, K.
AU - Banno, Yutaka
AU - Fujii, H.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Normal silkworms (Bombyx mori) have opaque larval skin due to uric acid accumulation in the epidermis while a mutant, og, is translucent owing to a deficiency in xanthine dehydrogenase (XDH), which synthesizes uric acid. Molybdenum cofactor (MoCo) sulfurase is responsible for XDH activation in various organisms. A silkworm MoCo sulfurase gene was cloned and found to be on the og locus, whose mutant alleles, ogk and ogt, show premature stop codons, proving that og is the MoCo sulfurase gene. It was observed that a miniature inverted-repeat transposable element (MITE), named Organdy, when inserted in an ogt mutant allele exon, causes unstable splicing of a downstream intron leading to incomplete open reading frames.
AB - Normal silkworms (Bombyx mori) have opaque larval skin due to uric acid accumulation in the epidermis while a mutant, og, is translucent owing to a deficiency in xanthine dehydrogenase (XDH), which synthesizes uric acid. Molybdenum cofactor (MoCo) sulfurase is responsible for XDH activation in various organisms. A silkworm MoCo sulfurase gene was cloned and found to be on the og locus, whose mutant alleles, ogk and ogt, show premature stop codons, proving that og is the MoCo sulfurase gene. It was observed that a miniature inverted-repeat transposable element (MITE), named Organdy, when inserted in an ogt mutant allele exon, causes unstable splicing of a downstream intron leading to incomplete open reading frames.
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UR - http://www.scopus.com/inward/citedby.url?scp=0242500384&partnerID=8YFLogxK
U2 - 10.1016/S0965-1748(03)00006-7
DO - 10.1016/S0965-1748(03)00006-7
M3 - Article
C2 - 12650690
AN - SCOPUS:0242500384
VL - 33
SP - 417
EP - 427
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
SN - 0965-1748
IS - 4
ER -