TY - JOUR
T1 - Mutations responsible for alcohol tolerance in the mutant of Synechococcus elongatus PCC 7942 (SY1043) obtained by single-cell screening system
AU - Hirokawa, Yasutaka
AU - Kanesaki, Yu
AU - Arai, Sayuri
AU - Saruta, Fumiko
AU - Hayashihara, Kayoko
AU - Murakami, Akio
AU - Shimizu, Kazunori
AU - Honda, Hiroyuki
AU - Yoshikawa, Hirofumi
AU - Hanai, Taizo
N1 - Funding Information:
This study was financially supported by the Core Research of Evolutional Science and Technology program (CREST) from the Japan Science and Technology Agency (JST). This study was also funded by the MEXT-supported Program for Strategic Research Foundation at Private Universities, 2013–2017 ( S1311017 ), and by Grant-in-Aid for Scientific Research (C) to YK ( 15K07368 ).
Publisher Copyright:
© 2017 The Society for Biotechnology, Japan
PY - 2018/5
Y1 - 2018/5
N2 - The production of alcohols directly from carbon dioxide by engineered cyanobacteria is an attractive technology for a sustainable future. Enhanced tolerance to the produced alcohols would be a desirable feature of the engineered cyanobacterial strains with higher alcohol productivity. We have recently obtained the mutant strains of Synechococcus elongatus PCC 7942 with higher tolerance to isopropanol using a single-cell screening system (Arai et al., Biotechnol. Bioeng., 114, 1771–1778, 2017). Among the mutant strains, SY1043 showed the highest isopropanol tolerance. Interestingly, SY1043 also showed higher tolerance to other alcohols such as ethanol and 1-butanol, however, the mechanisms involved in enhancing this alcohol tolerance were unclear. To reveal the alcohol tolerance mechanism of SY1043, we investigated the relationship between alcohol tolerance and four mutations found in SY1043 by genome resequencing analysis. Isopropanol tolerance was enhanced by amino acid substitution (Leu285Pro) in a hypothetical protein encoded by Synpcc7942_0180 of the wild type strain TA1297. TA4135, into which this mutation was introduced, showed a same tendency of tolerance to other alcohols (ethanol and 1-butanol).
AB - The production of alcohols directly from carbon dioxide by engineered cyanobacteria is an attractive technology for a sustainable future. Enhanced tolerance to the produced alcohols would be a desirable feature of the engineered cyanobacterial strains with higher alcohol productivity. We have recently obtained the mutant strains of Synechococcus elongatus PCC 7942 with higher tolerance to isopropanol using a single-cell screening system (Arai et al., Biotechnol. Bioeng., 114, 1771–1778, 2017). Among the mutant strains, SY1043 showed the highest isopropanol tolerance. Interestingly, SY1043 also showed higher tolerance to other alcohols such as ethanol and 1-butanol, however, the mechanisms involved in enhancing this alcohol tolerance were unclear. To reveal the alcohol tolerance mechanism of SY1043, we investigated the relationship between alcohol tolerance and four mutations found in SY1043 by genome resequencing analysis. Isopropanol tolerance was enhanced by amino acid substitution (Leu285Pro) in a hypothetical protein encoded by Synpcc7942_0180 of the wild type strain TA1297. TA4135, into which this mutation was introduced, showed a same tendency of tolerance to other alcohols (ethanol and 1-butanol).
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U2 - 10.1016/j.jbiosc.2017.11.012
DO - 10.1016/j.jbiosc.2017.11.012
M3 - Article
C2 - 29269101
AN - SCOPUS:85038377461
VL - 125
SP - 572
EP - 577
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -