MUTE Directly Orchestrates Cell-State Switch and the Single Symmetric Division to Create Stomata

Soon Ki Han, Xingyun Qi, Kei Sugihara, Jonathan H. Dang, Takaho A. Endo, Kristen L. Miller, Eun Deok Kim, Takashi Miura, Keiko U. Torii

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Precise cell division control is critical for developmental patterning. For the differentiation of a functional stoma, a cellular valve for efficient gas exchange, the single symmetric division of an immediate precursor is absolutely essential. Yet, the mechanism governing this event remains unclear. Here we report comprehensive inventories of gene expression by the Arabidopsis bHLH protein MUTE, a potent inducer of stomatal differentiation. MUTE switches the gene expression program initiated by SPEECHLESS. MUTE directly induces a suite of cell-cycle genes, including CYCD5;1, in which introduced expression triggers the symmetric divisions of arrested precursor cells in mute, and their transcriptional repressors, FAMA and FOUR LIPS. The regulatory network initiated by MUTE represents an incoherent type 1 feed-forward loop. Our mathematical modeling and experimental perturbations support a notion that MUTE orchestrates a transcriptional cascade leading to a tightly restricted pulse of cell-cycle gene expression, thereby ensuring the single cell division to create functional stomata. Stomata, small valves on the plant epidermis, are made of two guard cells surrounding a pore. Han et al. show that the transcription factor MUTE orchestrates gene regulatory circuits to switch cells to a differentiation state, then ensures that only a single symmetric division occurs to create a functional stoma.

Original languageEnglish
Pages (from-to)303-315.e5
JournalDevelopmental Cell
Volume45
Issue number3
DOIs
Publication statusPublished - May 7 2018

Fingerprint

cdc Genes
Cells
Switches
Gene expression
Gene Expression
Cell Division
Plant Epidermis
Switch Genes
Arabidopsis Proteins
Basic Helix-Loop-Helix Transcription Factors
Genes
Gene Regulatory Networks
Transcription Factors
Gases
Equipment and Supplies
Networks (circuits)

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Cite this

Han, S. K., Qi, X., Sugihara, K., Dang, J. H., Endo, T. A., Miller, K. L., ... Torii, K. U. (2018). MUTE Directly Orchestrates Cell-State Switch and the Single Symmetric Division to Create Stomata. Developmental Cell, 45(3), 303-315.e5. https://doi.org/10.1016/j.devcel.2018.04.010

MUTE Directly Orchestrates Cell-State Switch and the Single Symmetric Division to Create Stomata. / Han, Soon Ki; Qi, Xingyun; Sugihara, Kei; Dang, Jonathan H.; Endo, Takaho A.; Miller, Kristen L.; Kim, Eun Deok; Miura, Takashi; Torii, Keiko U.

In: Developmental Cell, Vol. 45, No. 3, 07.05.2018, p. 303-315.e5.

Research output: Contribution to journalArticle

Han, SK, Qi, X, Sugihara, K, Dang, JH, Endo, TA, Miller, KL, Kim, ED, Miura, T & Torii, KU 2018, 'MUTE Directly Orchestrates Cell-State Switch and the Single Symmetric Division to Create Stomata', Developmental Cell, vol. 45, no. 3, pp. 303-315.e5. https://doi.org/10.1016/j.devcel.2018.04.010
Han, Soon Ki ; Qi, Xingyun ; Sugihara, Kei ; Dang, Jonathan H. ; Endo, Takaho A. ; Miller, Kristen L. ; Kim, Eun Deok ; Miura, Takashi ; Torii, Keiko U. / MUTE Directly Orchestrates Cell-State Switch and the Single Symmetric Division to Create Stomata. In: Developmental Cell. 2018 ; Vol. 45, No. 3. pp. 303-315.e5.
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