Abstract
Among non-tuberculous mycobacteria (NTM), the Mycobacterium simiae complex is one of the largest groups, consisting of 18 species of slow-growing mycobacteria. In 2009, a case of NTM-associated infectious skin disease was reported in Shiga Prefecture, Japan. The patient presented with scattered nodules on the chest, back and extremities, and an M. simiae-like organism was isolated from skin biopsy specimens obtained from one of these lesions. Based on several assessments, including multiple-gene analyses, biochemical characterization and drug susceptibility testing, we concluded that this isolate represented a novel species of NTM, and proposed the name ‘Mycobacterium shigaense’. Since 2009, five more cases of NTM-associated infectious disease in which there was a suspected involvement of ‘M. shigaense’ have been reported. Interestingly, four of these six cases occurred in Shiga Prefecture. Here we performed multiple-gene phylogenetic analyses, physiological and biochemical characterization tests, drug susceptibility tests, and profiling of proteins, fatty acids and mycolic acids of eight clinical isolates from the six suspected ‘M. shigaense’ cases. The results confirmed that all of the clinical isolates were ‘M. shigaense’, a slow-growing, scotochromogenic species. Here M. shigaense is validly proposed as a new member of the M. simiae complex, with the type strain being UN-152T (=JCM 32072T =DSM 46748T).
| Original language | English |
|---|---|
| Article number | 002845 |
| Pages (from-to) | 2437-2442 |
| Number of pages | 6 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 68 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 1 2018 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Mycobacterium shigaense sp. Nov., a slow-growing, scotochromogenic species, is a member of the mycobacterium simiae complex. / Fukano, Hanako; Yoshida, Mitsunori; Kazumi, Yuko; Fujiwara, Nagatoshi; Katayama, Kinya; Ogura, Yoshitoshi; Hayashi, Tetsuya; Miyamoto, Yuji; Fujimoto, Noriki; Hongsheng, Wang; Mizumoto, Chisaki; Koizumi, Yusuke; Maeda, Hiroyoshi; Hiranuma, Osamu; Mitarai, Satoshi; Ishii, Norihisa; Hoshino, Yoshihiko.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 8, 002845, 01.08.2018, p. 2437-2442.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Mycobacterium shigaense sp. Nov., a slow-growing, scotochromogenic species, is a member of the mycobacterium simiae complex
AU - Fukano, Hanako
AU - Yoshida, Mitsunori
AU - Kazumi, Yuko
AU - Fujiwara, Nagatoshi
AU - Katayama, Kinya
AU - Ogura, Yoshitoshi
AU - Hayashi, Tetsuya
AU - Miyamoto, Yuji
AU - Fujimoto, Noriki
AU - Hongsheng, Wang
AU - Mizumoto, Chisaki
AU - Koizumi, Yusuke
AU - Maeda, Hiroyoshi
AU - Hiranuma, Osamu
AU - Mitarai, Satoshi
AU - Ishii, Norihisa
AU - Hoshino, Yoshihiko
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Among non-tuberculous mycobacteria (NTM), the Mycobacterium simiae complex is one of the largest groups, consisting of 18 species of slow-growing mycobacteria. In 2009, a case of NTM-associated infectious skin disease was reported in Shiga Prefecture, Japan. The patient presented with scattered nodules on the chest, back and extremities, and an M. simiae-like organism was isolated from skin biopsy specimens obtained from one of these lesions. Based on several assessments, including multiple-gene analyses, biochemical characterization and drug susceptibility testing, we concluded that this isolate represented a novel species of NTM, and proposed the name ‘Mycobacterium shigaense’. Since 2009, five more cases of NTM-associated infectious disease in which there was a suspected involvement of ‘M. shigaense’ have been reported. Interestingly, four of these six cases occurred in Shiga Prefecture. Here we performed multiple-gene phylogenetic analyses, physiological and biochemical characterization tests, drug susceptibility tests, and profiling of proteins, fatty acids and mycolic acids of eight clinical isolates from the six suspected ‘M. shigaense’ cases. The results confirmed that all of the clinical isolates were ‘M. shigaense’, a slow-growing, scotochromogenic species. Here M. shigaense is validly proposed as a new member of the M. simiae complex, with the type strain being UN-152T (=JCM 32072T =DSM 46748T).
AB - Among non-tuberculous mycobacteria (NTM), the Mycobacterium simiae complex is one of the largest groups, consisting of 18 species of slow-growing mycobacteria. In 2009, a case of NTM-associated infectious skin disease was reported in Shiga Prefecture, Japan. The patient presented with scattered nodules on the chest, back and extremities, and an M. simiae-like organism was isolated from skin biopsy specimens obtained from one of these lesions. Based on several assessments, including multiple-gene analyses, biochemical characterization and drug susceptibility testing, we concluded that this isolate represented a novel species of NTM, and proposed the name ‘Mycobacterium shigaense’. Since 2009, five more cases of NTM-associated infectious disease in which there was a suspected involvement of ‘M. shigaense’ have been reported. Interestingly, four of these six cases occurred in Shiga Prefecture. Here we performed multiple-gene phylogenetic analyses, physiological and biochemical characterization tests, drug susceptibility tests, and profiling of proteins, fatty acids and mycolic acids of eight clinical isolates from the six suspected ‘M. shigaense’ cases. The results confirmed that all of the clinical isolates were ‘M. shigaense’, a slow-growing, scotochromogenic species. Here M. shigaense is validly proposed as a new member of the M. simiae complex, with the type strain being UN-152T (=JCM 32072T =DSM 46748T).
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UR - http://www.scopus.com/inward/citedby.url?scp=85048928309&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002845
DO - 10.1099/ijsem.0.002845
M3 - Article
VL - 68
SP - 2437
EP - 2442
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 8
M1 - 002845
ER -