Mycobacterium shigaense sp. Nov., a slow-growing, scotochromogenic species, is a member of the mycobacterium simiae complex

Hanako Fukano, Mitsunori Yoshida, Yuko Kazumi, Nagatoshi Fujiwara, Kinya Katayama, Yoshitoshi Ogura, Tetsuya Hayashi, Yuji Miyamoto, Noriki Fujimoto, Wang Hongsheng, Chisaki Mizumoto, Yusuke Koizumi, Hiroyoshi Maeda, Osamu Hiranuma, Satoshi Mitarai, Norihisa Ishii, Yoshihiko Hoshino

Research output: Contribution to journalArticle

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Abstract

Among non-tuberculous mycobacteria (NTM), the Mycobacterium simiae complex is one of the largest groups, consisting of 18 species of slow-growing mycobacteria. In 2009, a case of NTM-associated infectious skin disease was reported in Shiga Prefecture, Japan. The patient presented with scattered nodules on the chest, back and extremities, and an M. simiae-like organism was isolated from skin biopsy specimens obtained from one of these lesions. Based on several assessments, including multiple-gene analyses, biochemical characterization and drug susceptibility testing, we concluded that this isolate represented a novel species of NTM, and proposed the name ‘Mycobacterium shigaense’. Since 2009, five more cases of NTM-associated infectious disease in which there was a suspected involvement of ‘M. shigaense’ have been reported. Interestingly, four of these six cases occurred in Shiga Prefecture. Here we performed multiple-gene phylogenetic analyses, physiological and biochemical characterization tests, drug susceptibility tests, and profiling of proteins, fatty acids and mycolic acids of eight clinical isolates from the six suspected ‘M. shigaense’ cases. The results confirmed that all of the clinical isolates were ‘M. shigaense’, a slow-growing, scotochromogenic species. Here M. shigaense is validly proposed as a new member of the M. simiae complex, with the type strain being UN-152T (=JCM 32072T =DSM 46748T).

Original languageEnglish
Article number002845
Pages (from-to)2437-2442
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number8
DOIs
Publication statusPublished - Aug 1 2018

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Mycobacterium simiae
Mycobacterium
skin
drug
gene
infectious disease
lesion
fatty acid
Infectious Skin Diseases
phylogenetics
drugs
Mycolic Acids
protein
acid
testing
skin diseases
chest
United Nations
infectious diseases
biopsy

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Mycobacterium shigaense sp. Nov., a slow-growing, scotochromogenic species, is a member of the mycobacterium simiae complex. / Fukano, Hanako; Yoshida, Mitsunori; Kazumi, Yuko; Fujiwara, Nagatoshi; Katayama, Kinya; Ogura, Yoshitoshi; Hayashi, Tetsuya; Miyamoto, Yuji; Fujimoto, Noriki; Hongsheng, Wang; Mizumoto, Chisaki; Koizumi, Yusuke; Maeda, Hiroyoshi; Hiranuma, Osamu; Mitarai, Satoshi; Ishii, Norihisa; Hoshino, Yoshihiko.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 8, 002845, 01.08.2018, p. 2437-2442.

Research output: Contribution to journalArticle

Fukano, H, Yoshida, M, Kazumi, Y, Fujiwara, N, Katayama, K, Ogura, Y, Hayashi, T, Miyamoto, Y, Fujimoto, N, Hongsheng, W, Mizumoto, C, Koizumi, Y, Maeda, H, Hiranuma, O, Mitarai, S, Ishii, N & Hoshino, Y 2018, 'Mycobacterium shigaense sp. Nov., a slow-growing, scotochromogenic species, is a member of the mycobacterium simiae complex', International Journal of Systematic and Evolutionary Microbiology, vol. 68, no. 8, 002845, pp. 2437-2442. https://doi.org/10.1099/ijsem.0.002845
Fukano, Hanako ; Yoshida, Mitsunori ; Kazumi, Yuko ; Fujiwara, Nagatoshi ; Katayama, Kinya ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Miyamoto, Yuji ; Fujimoto, Noriki ; Hongsheng, Wang ; Mizumoto, Chisaki ; Koizumi, Yusuke ; Maeda, Hiroyoshi ; Hiranuma, Osamu ; Mitarai, Satoshi ; Ishii, Norihisa ; Hoshino, Yoshihiko. / Mycobacterium shigaense sp. Nov., a slow-growing, scotochromogenic species, is a member of the mycobacterium simiae complex. In: International Journal of Systematic and Evolutionary Microbiology. 2018 ; Vol. 68, No. 8. pp. 2437-2442.
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AU - Katayama, Kinya

AU - Ogura, Yoshitoshi

AU - Hayashi, Tetsuya

AU - Miyamoto, Yuji

AU - Fujimoto, Noriki

AU - Hongsheng, Wang

AU - Mizumoto, Chisaki

AU - Koizumi, Yusuke

AU - Maeda, Hiroyoshi

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AU - Mitarai, Satoshi

AU - Ishii, Norihisa

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