MyD88-dependent signaling for IL-15 production plays an important role in maintenance of CD8αα TCRαβ and TCRγδ intestinal intraepithelial lymphocytes

Qingsheng Yu, Ce Tang, Sun Xun, Toshiki Yajima, Kiyoshi Takeda, Yasunobu Yoshikai

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    58 Citations (Scopus)

    Abstract

    Interaction between commensal bacteria and intestinal epithelial cells (i-ECs) via TLRs is important for intestinal homeostasis. In this study, we found that the numbers of CB8αα TCRαβ and TCRγδ intestinal intraepithelial lymphocytes (i-IELs) were significantly decreased in MyD88-deficient (-/-) mice. The expression of IL-15 by i-ECs was severely reduced in MyD88-/- mice. Introduction of IL-15 transgene into MyD88-/- mice (MyD88-/- IL-15 transgenic mice) partly restored the numbers of CD8αα TCRαβ and TCRγδ i-IELs. The i-IEL in irradiated wild-type (WT) mice transferred with MyD88-/- bone marrow (BM) cells had the same proportions of i-IEL as WT mice, whereas those in irradiated MyD88-/- mice transferred with WT BM cells showed significantly reduced proportions of CD8αα TCRαβ and TCRγδ i-IELs, as was similar to the proportions found in MyD88-/- mice. However, irradiated MyD88-/- IL-15 transgenic mice transferred with WT BM cells had increased numbers of CD8αα TCRαβ and TCRγδ subsets in the i-IEL. These results suggest that parenchymal cells such as i-ECs contribute to the maintenance of CD8αα TCRαβ and γδ i-IELs at least partly via MyD88-dependent IL-15 production.

    Original languageEnglish
    Pages (from-to)6180-6185
    Number of pages6
    JournalJournal of Immunology
    Volume176
    Issue number10
    DOIs
    Publication statusPublished - May 15 2006

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

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