MyD88-dependent signaling for IL-15 production plays an important role in maintenance of CD8αα TCRαβ and TCRγδ intestinal intraepithelial lymphocytes

Qingsheng Yu, Ce Tang, Sun Xun, Toshiki Yajima, Kiyoshi Takeda, Yasunobu Yoshikai

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    Abstract

    Interaction between commensal bacteria and intestinal epithelial cells (i-ECs) via TLRs is important for intestinal homeostasis. In this study, we found that the numbers of CB8αα TCRαβ and TCRγδ intestinal intraepithelial lymphocytes (i-IELs) were significantly decreased in MyD88-deficient (-/-) mice. The expression of IL-15 by i-ECs was severely reduced in MyD88-/- mice. Introduction of IL-15 transgene into MyD88-/- mice (MyD88-/- IL-15 transgenic mice) partly restored the numbers of CD8αα TCRαβ and TCRγδ i-IELs. The i-IEL in irradiated wild-type (WT) mice transferred with MyD88-/- bone marrow (BM) cells had the same proportions of i-IEL as WT mice, whereas those in irradiated MyD88-/- mice transferred with WT BM cells showed significantly reduced proportions of CD8αα TCRαβ and TCRγδ i-IELs, as was similar to the proportions found in MyD88-/- mice. However, irradiated MyD88-/- IL-15 transgenic mice transferred with WT BM cells had increased numbers of CD8αα TCRαβ and TCRγδ subsets in the i-IEL. These results suggest that parenchymal cells such as i-ECs contribute to the maintenance of CD8αα TCRαβ and γδ i-IELs at least partly via MyD88-dependent IL-15 production.

    Original languageEnglish
    Pages (from-to)6180-6185
    Number of pages6
    JournalJournal of Immunology
    Volume176
    Issue number10
    Publication statusPublished - May 15 2006

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    Interleukin-15
    Maintenance
    Lymphocytes
    Bone Marrow Cells
    Epithelial Cells
    Transgenic Mice
    Transgenes
    Homeostasis
    Bacteria

    All Science Journal Classification (ASJC) codes

    • Immunology

    Cite this

    MyD88-dependent signaling for IL-15 production plays an important role in maintenance of CD8αα TCRαβ and TCRγδ intestinal intraepithelial lymphocytes. / Yu, Qingsheng; Tang, Ce; Xun, Sun; Yajima, Toshiki; Takeda, Kiyoshi; Yoshikai, Yasunobu.

    In: Journal of Immunology, Vol. 176, No. 10, 15.05.2006, p. 6180-6185.

    Research output: Contribution to journalArticle

    Yu, Qingsheng ; Tang, Ce ; Xun, Sun ; Yajima, Toshiki ; Takeda, Kiyoshi ; Yoshikai, Yasunobu. / MyD88-dependent signaling for IL-15 production plays an important role in maintenance of CD8αα TCRαβ and TCRγδ intestinal intraepithelial lymphocytes. In: Journal of Immunology. 2006 ; Vol. 176, No. 10. pp. 6180-6185.
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    abstract = "Interaction between commensal bacteria and intestinal epithelial cells (i-ECs) via TLRs is important for intestinal homeostasis. In this study, we found that the numbers of CB8αα TCRαβ and TCRγδ intestinal intraepithelial lymphocytes (i-IELs) were significantly decreased in MyD88-deficient (-/-) mice. The expression of IL-15 by i-ECs was severely reduced in MyD88-/- mice. Introduction of IL-15 transgene into MyD88-/- mice (MyD88-/- IL-15 transgenic mice) partly restored the numbers of CD8αα TCRαβ and TCRγδ i-IELs. The i-IEL in irradiated wild-type (WT) mice transferred with MyD88-/- bone marrow (BM) cells had the same proportions of i-IEL as WT mice, whereas those in irradiated MyD88-/- mice transferred with WT BM cells showed significantly reduced proportions of CD8αα TCRαβ and TCRγδ i-IELs, as was similar to the proportions found in MyD88-/- mice. However, irradiated MyD88-/- IL-15 transgenic mice transferred with WT BM cells had increased numbers of CD8αα TCRαβ and TCRγδ subsets in the i-IEL. These results suggest that parenchymal cells such as i-ECs contribute to the maintenance of CD8αα TCRαβ and γδ i-IELs at least partly via MyD88-dependent IL-15 production.",
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