Myeloid or lymphoid promiscuity as a critical step in hematopoietic lineage commitment

Toshihiro Miyamoto, Hiromi Iwasaki, Boris Reizis, Min Ye, Thomas Graf, Irving L. Weissman, Koichi Akashi

Research output: Contribution to journalArticlepeer-review

358 Citations (Scopus)

Abstract

We demonstrate here that "promiscuous" expression of myeloid or lymphoid genes precedes lineage commitment in hematopoiesis. Prospectively purified single common myeloid progenitors (CMPs) coexpress myelo-erythroid but not lymphoid genes, whereas single common lymphoid progenitors (CLPs) coexpress T and B lymphoid but not myeloid genes. Genes unrelated to the adopted lineage are downregulated in bipotent and monopotent descendants of CMPs and CLPs. Promiscuous gene expression does not alter the biological potential of multipotent progenitors: CMPs with an activated endogenous M lysozyme locus yield normal proportions of myelo-erythroid colonies, and CLPs expressing the pre-T cell receptor α gene differentiate into normal numbers of B cells. Thus, the accessibility for multiple myeloid or lymphoid programs promiscuously may allow flexibility in fate commitments at these multipotent stages.

Original languageEnglish
Pages (from-to)137-147
Number of pages11
JournalDevelopmental Cell
Volume3
Issue number1
DOIs
Publication statusPublished - 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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