Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function

Chandrashekara Mallappa, Brian T. Nasipak, Letitiah Etheridge, Elliot J. Androphy, Stephen N. Jones, Charles G. Sagerström, Yasuyuki Ohkawa, Anthony N. Imbalzano

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.

Original languageEnglish
Pages (from-to)3176-3186
Number of pages11
JournalMolecular and cellular biology
Issue number13
Publication statusPublished - Jul 2010

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function'. Together they form a unique fingerprint.

Cite this