Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis

Yasuyuki Ohkawa, Saori Yoshimura, Chiduru Higashi, Concetta G.A. Marfella, Caroline S. Dacwag, Taro Tachibana, Anthony N. Imbalzano

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Many studies have examined transcriptional regulation during the initiation of skeletal muscle differentiation; however, there is less information regarding transcriptional control during adult myogenesis and during the maintenance of the differentiated state. MyoD and the mammalian SWI/SNF chromatin-remodeling enzymes containing the Brg1 ATPase are necessary to induce myogenesis in cell culture models and in developing embryonic tissue, whereas myogenin and Brg1 are critical for the expression of the late genes that induce terminal muscle differentiation. Here, we demonstrate that myogenin also binds to its own promoter during the late stages of embryonic muscle development. As is the case during embryonic myogenesis, MyoD and Brg1 co-localize to the myogenin promoter in primary adult muscle satellite cells. However, in mature myofibers, myogenin and Brg1 are preferentially co-localized to the myogenin promoter. Thus, the myogenin promoter is occupied by different myogenic factors at different times of myogenesis. The relevance of myogenin in the continued expression from its own promoter is demonstrated in culture, where we show that myogenin, in the absence of MyoD, is capable of maintaining its own expression by recruiting the Brg1 ATPase to modify promoter chromatin structure and facilitate myogenin expression. Finally, we utilized in vivo electroporation to demonstrate that Brg1 is required for the continued production of the myogenin protein in newborn skeletal muscle tissue. These findings strongly suggest that the skeletal muscle phenotype is maintained by myogenin and the continuous activity of Brg1-based SWI/SNF chromatin-remodeling enzymes.

Original languageEnglish
Pages (from-to)6564-6570
Number of pages7
JournalJournal of Biological Chemistry
Volume282
Issue number9
DOIs
Publication statusPublished - Mar 2 2007
Externally publishedYes

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Myogenin
Muscle Development
Gene expression
Adenosine Triphosphatases
Gene Expression
Muscle
Chromatin
Skeletal Muscle
Chromatin Assembly and Disassembly
Tissue
Muscles
Electroporation
Enzymes
Cell culture
Muscle Cells
Embryonic Development
Cell Culture Techniques
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ohkawa, Y., Yoshimura, S., Higashi, C., Marfella, C. G. A., Dacwag, C. S., Tachibana, T., & Imbalzano, A. N. (2007). Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis. Journal of Biological Chemistry, 282(9), 6564-6570. https://doi.org/10.1074/jbc.M608898200

Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis. / Ohkawa, Yasuyuki; Yoshimura, Saori; Higashi, Chiduru; Marfella, Concetta G.A.; Dacwag, Caroline S.; Tachibana, Taro; Imbalzano, Anthony N.

In: Journal of Biological Chemistry, Vol. 282, No. 9, 02.03.2007, p. 6564-6570.

Research output: Contribution to journalArticle

Ohkawa, Y, Yoshimura, S, Higashi, C, Marfella, CGA, Dacwag, CS, Tachibana, T & Imbalzano, AN 2007, 'Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis', Journal of Biological Chemistry, vol. 282, no. 9, pp. 6564-6570. https://doi.org/10.1074/jbc.M608898200
Ohkawa, Yasuyuki ; Yoshimura, Saori ; Higashi, Chiduru ; Marfella, Concetta G.A. ; Dacwag, Caroline S. ; Tachibana, Taro ; Imbalzano, Anthony N. / Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 9. pp. 6564-6570.
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