Myosin-II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis

Zhonghui Feng, Satoshi Okada, Guoping Cai, Bing Zhou, Erfei Bi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

MLC1 is a haploinsufficient gene encoding the essential light chain for Myo1, the sole myosin-II heavy chain in the budding yeast Saccharomyces cerevisiae. Mlc1 defines an essential hub that coordinates actomyosin ring function, membrane trafficking, and septum formation during cytokinesis by binding to IQGAP, myosin-II, and myosin-V. However, the mechanism of how Mlc1 is targeted to the division site during the cell cycle remains unsolved. By constructing a GFP-tagged MLC1 under its own promoter control and using quantitative live-cell imaging coupled with yeast mutants, we found that septin ring and actin filaments mediate the targeting of Mlc1 to the division site before and during cytokinesis, respectively. Both mechanisms contribute to and are collectively required for the accumulation of Mlc1 at the division site during cytokinesis. We also found that Myo1 plays a major role in the septin-dependent Mlc1 localization before cytokinesis, whereas the formin Bni1 plays a major role in the actin filament-dependent Mlc1 localization during cytokinesis. Such a two-tiered mechanism for Mlc1 localization is presumably required for the ordered assembly and robustness of cytokinesis machinery and is likely conserved across species.

Original languageEnglish
Pages (from-to)1211-1224
Number of pages14
JournalMolecular biology of the cell
Volume26
Issue number7
DOIs
Publication statusPublished - Apr 1 2015

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Myosin Type II
Myosin Light Chains
Cytokinesis
Myosin Heavy Chains
Septins
Actin Cytoskeleton
Myosin Type V
Actomyosin
Saccharomycetales
Essential Genes
Saccharomyces cerevisiae
Cell Cycle
Yeasts
Light
Membranes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Myosin-II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis. / Feng, Zhonghui; Okada, Satoshi; Cai, Guoping; Zhou, Bing; Bi, Erfei.

In: Molecular biology of the cell, Vol. 26, No. 7, 01.04.2015, p. 1211-1224.

Research output: Contribution to journalArticle

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AU - Cai, Guoping

AU - Zhou, Bing

AU - Bi, Erfei

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