TY - JOUR
T1 - Myricetin improves cognitive function in SAMP8 mice and upregulates brain-derived neurotrophic factor and nerve growth factor
AU - Shimada, Yu
AU - Sato, Yuka
AU - Kumazoe, Motofumi
AU - Kitamura, Ryo
AU - Fujimura, Yoshinori
AU - Tachibana, Hirofumi
N1 - Funding Information:
This work was supported in part by JSPS KAKENHI grants JP20H05683 and JP15H02448 to H. Tachibana. And the JSPS KAKENHI grant JP20K05960 to M Kumazoe. We appreciate the technical assistance from the Research Support Centre, Research Canter for Human Disease Modelling, Kyushu University Graduate School of Medical Sciences, and from the Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University .
Funding Information:
This work was supported in part by JSPS KAKENHI grants JP20H05683 and JP15H02448 to H. Tachibana. And the JSPS KAKENHI grant JP20K05960 to M Kumazoe. We appreciate the technical assistance from the Research Support Centre, Research Canter for Human Disease Modelling, Kyushu University Graduate School of Medical Sciences, and from the Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University.
Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2022/8/6
Y1 - 2022/8/6
N2 - Introduction: Considering that neurodegeneration is an irreversible process, an efficient, low-burden approach to prevent dementia is strongly needed. Here, we show that the daily intake of myricetin normalised cognitive dysfunction in senescence-accelerated mouse prone 8 (SAMP8) mice. Methods: SAMP8 mice were fed a diet supplemented with myricetin and novel object recognition tests and Y-maze tests were performed. Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in brains of SAMP8 mice were measured. The phosphorylation level of cAMP-response-element-binding protein (CREB) level in brains of SAMP8 mice were evaluated. Also, SH-SY5Y cells were treated with myricetin and cAMP levels were measured. Results: In SAMP8 mice, neurotrophins, including BDNF and NGF, were downregulated relative to levels in their normal counterparts. In addition, myricetin intake upregulated the phosphorylation of CREB, the major transcription factor for BDNF and NGF. Also, myricetin induced cAMP upregulation, and CREB phosphorylation via a cAMP-dependent protein kinase-dependent manner in SH-SY5Y cells. Conclusion: Taken together, myricetin improves cognitive function in SAMP8 mice and upregulates BDNF and NGF.
AB - Introduction: Considering that neurodegeneration is an irreversible process, an efficient, low-burden approach to prevent dementia is strongly needed. Here, we show that the daily intake of myricetin normalised cognitive dysfunction in senescence-accelerated mouse prone 8 (SAMP8) mice. Methods: SAMP8 mice were fed a diet supplemented with myricetin and novel object recognition tests and Y-maze tests were performed. Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in brains of SAMP8 mice were measured. The phosphorylation level of cAMP-response-element-binding protein (CREB) level in brains of SAMP8 mice were evaluated. Also, SH-SY5Y cells were treated with myricetin and cAMP levels were measured. Results: In SAMP8 mice, neurotrophins, including BDNF and NGF, were downregulated relative to levels in their normal counterparts. In addition, myricetin intake upregulated the phosphorylation of CREB, the major transcription factor for BDNF and NGF. Also, myricetin induced cAMP upregulation, and CREB phosphorylation via a cAMP-dependent protein kinase-dependent manner in SH-SY5Y cells. Conclusion: Taken together, myricetin improves cognitive function in SAMP8 mice and upregulates BDNF and NGF.
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U2 - 10.1016/j.bbrc.2022.05.039
DO - 10.1016/j.bbrc.2022.05.039
M3 - Article
C2 - 35636253
AN - SCOPUS:85130558125
SN - 0006-291X
VL - 616
SP - 33
EP - 40
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
ER -