N-glycosylation with synthetic undecaprenyl pyrophosphate-linked oligosaccharide to oligopeptides by PglB oligosaccharyltransferase from Campylobacter jejuni

Akihiro Ishiwata, Yuya Taguchi, Yong Joo Lee, Taisuke Watanabe, Daisuke Kohda, Yukishige Ito

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    The oligosaccharyltransferase PglB from Campylobacter jejuni catalyses the N-glycosylation reaction with undecaprenyl-pyrophosphate-linked Glc1GalNAc5Bac1 (Und-PP-Glc1GalNAc5Bac1). Experiments using chemically synthesized donors coupled to fluorescently tagged peptides confirmed that biosynthetic intermediate Und-PP-Bac1 and Und-PP-GalNAc2Bac1 are transferred efficiently to the Asn residue in the consensus sequence (D/E-X'-N-X-T/S, X',X≠P). The products were analyzed in detail by tandem MS to confirm their chemical structures.

    Original languageEnglish
    Pages (from-to)731-737
    Number of pages7
    JournalChemBioChem
    Volume16
    Issue number5
    DOIs
    Publication statusPublished - Mar 23 2015

    Fingerprint

    Glycosylation
    Oligopeptides
    Campylobacter jejuni
    Consensus Sequence
    Oligosaccharides
    Peptides
    Experiments
    dolichyl-diphosphooligosaccharide - protein glycotransferase
    undecaprenyl pyrophosphate

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Organic Chemistry

    Cite this

    N-glycosylation with synthetic undecaprenyl pyrophosphate-linked oligosaccharide to oligopeptides by PglB oligosaccharyltransferase from Campylobacter jejuni. / Ishiwata, Akihiro; Taguchi, Yuya; Lee, Yong Joo; Watanabe, Taisuke; Kohda, Daisuke; Ito, Yukishige.

    In: ChemBioChem, Vol. 16, No. 5, 23.03.2015, p. 731-737.

    Research output: Contribution to journalArticle

    Ishiwata, Akihiro ; Taguchi, Yuya ; Lee, Yong Joo ; Watanabe, Taisuke ; Kohda, Daisuke ; Ito, Yukishige. / N-glycosylation with synthetic undecaprenyl pyrophosphate-linked oligosaccharide to oligopeptides by PglB oligosaccharyltransferase from Campylobacter jejuni. In: ChemBioChem. 2015 ; Vol. 16, No. 5. pp. 731-737.
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    abstract = "The oligosaccharyltransferase PglB from Campylobacter jejuni catalyses the N-glycosylation reaction with undecaprenyl-pyrophosphate-linked Glc1GalNAc5Bac1 (Und-PP-Glc1GalNAc5Bac1). Experiments using chemically synthesized donors coupled to fluorescently tagged peptides confirmed that biosynthetic intermediate Und-PP-Bac1 and Und-PP-GalNAc2Bac1 are transferred efficiently to the Asn residue in the consensus sequence (D/E-X'-N-X-T/S, X',X≠P). The products were analyzed in detail by tandem MS to confirm their chemical structures.",
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    AU - Taguchi, Yuya

    AU - Lee, Yong Joo

    AU - Watanabe, Taisuke

    AU - Kohda, Daisuke

    AU - Ito, Yukishige

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