NADH:ferredoxin reductase and NAD-reducing hydrogenase activities in Hydrogenobacter thermophilus strain TK-6

Ki Seok Yoon, Yasufumi Ueda, Masaharu Ishii, Yasuo Igarashi, Tohru Kodama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

NADH:ferredoxin reductase (EC 1.18.13) and NAD-reducing hydrogenase (EC 1.12.1.2) activities were detected in the cytoplasm of Hydrogenobacter thermophilus TK-6. NADH:ferredoxin reductase activity was detected using metronidazole, an artificial electron acceptor, which reacts specifica1ly with reduced ferredoxin. Soluble NAD-reducing hydrogenase activity was detected after extended preincubation. The lag disappeared when cell-free extract was incubated anaerobically for more than 30 min. The electron transport system of this chemolithoautotrophic bacterium is discussed.

Original languageEnglish
Pages (from-to)139-142
Number of pages4
JournalFEMS microbiology letters
Volume139
Issue number2-3
DOIs
Publication statusPublished - Jun 1 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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