NADH2- and succinate-dependent O2 uptake in chromatophores from chromatium vinosum

Ken Ichiro Takamiya, Keiko Kimura, Michio Doi, Mitsuo Nishimura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The O2 uptake linked to NADH2 and succinate oxidation was observed in chromatophores from photosynthetically grown Chromatium vinosum.The maximal rate was 60-120 nmoles of O2 uptake per min per ̈mole of bacterio-chlorophyll. The rate of O2 uptake linked to NADH2 oxidation was higher in the neutral-to-acidic pH range than in the alkaline range, whereas that linked to succinate oxidation was higher in the alkaline range.The O2 uptake linked to NADH2 oxidation was inhibited by rotenone, amytal, antimycin A, KCN and NaN3, while that linked to succinate oxidation was inhibited by antimycin A, KCN and NaN3. Malate, citrate, pyruvate, acetate, α-ketoglutarate, NADPH2 and thiosulfate did not serve as substrates for the O2 uptake of isolated chromatophores.The rates of the O2 uptake linked to both NADH2 and succinate oxidation were not stimulated by adding uncouplers or under phosphorylating conditions. Little or no ATP was synthesized in the dark, coupled to either NADH2 or succinate oxidation, in spite of a high activity of photophosphorylation in Chromatium chromatophores.

Original languageEnglish
Pages (from-to)405-411
Number of pages7
JournalPlant and Cell Physiology
Volume21
Issue number3
Publication statusPublished - May 1 1980

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Allochromatium vinosum
Chromatium
Chromatophores
chromatophores
Succinic Acid
succinic acid
oxidation
Antimycin A
Sodium Azide
antimycin A
Photophosphorylation
Amobarbital
Rotenone
Thiosulfates
Chlorophyll
Pyruvic Acid
Citric Acid
photophosphorylation
rotenone
thiosulfates

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Takamiya, K. I., Kimura, K., Doi, M., & Nishimura, M. (1980). NADH2- and succinate-dependent O2 uptake in chromatophores from chromatium vinosum. Plant and Cell Physiology, 21(3), 405-411.

NADH2- and succinate-dependent O2 uptake in chromatophores from chromatium vinosum. / Takamiya, Ken Ichiro; Kimura, Keiko; Doi, Michio; Nishimura, Mitsuo.

In: Plant and Cell Physiology, Vol. 21, No. 3, 01.05.1980, p. 405-411.

Research output: Contribution to journalArticle

Takamiya, KI, Kimura, K, Doi, M & Nishimura, M 1980, 'NADH2- and succinate-dependent O2 uptake in chromatophores from chromatium vinosum', Plant and Cell Physiology, vol. 21, no. 3, pp. 405-411.
Takamiya KI, Kimura K, Doi M, Nishimura M. NADH2- and succinate-dependent O2 uptake in chromatophores from chromatium vinosum. Plant and Cell Physiology. 1980 May 1;21(3):405-411.
Takamiya, Ken Ichiro ; Kimura, Keiko ; Doi, Michio ; Nishimura, Mitsuo. / NADH2- and succinate-dependent O2 uptake in chromatophores from chromatium vinosum. In: Plant and Cell Physiology. 1980 ; Vol. 21, No. 3. pp. 405-411.
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AB - The O2 uptake linked to NADH2 and succinate oxidation was observed in chromatophores from photosynthetically grown Chromatium vinosum.The maximal rate was 60-120 nmoles of O2 uptake per min per ̈mole of bacterio-chlorophyll. The rate of O2 uptake linked to NADH2 oxidation was higher in the neutral-to-acidic pH range than in the alkaline range, whereas that linked to succinate oxidation was higher in the alkaline range.The O2 uptake linked to NADH2 oxidation was inhibited by rotenone, amytal, antimycin A, KCN and NaN3, while that linked to succinate oxidation was inhibited by antimycin A, KCN and NaN3. Malate, citrate, pyruvate, acetate, α-ketoglutarate, NADPH2 and thiosulfate did not serve as substrates for the O2 uptake of isolated chromatophores.The rates of the O2 uptake linked to both NADH2 and succinate oxidation were not stimulated by adding uncouplers or under phosphorylating conditions. Little or no ATP was synthesized in the dark, coupled to either NADH2 or succinate oxidation, in spite of a high activity of photophosphorylation in Chromatium chromatophores.

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