TY - JOUR
T1 - Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications
AU - Hastuti, Novitri
AU - Darmayanti, Rizki Fitria
AU - Hardiningtyas, Safrina Dyah
AU - Kanomata, Kyohei
AU - Sonomoto, Kenji
AU - Goto, Masahiro
AU - Kitaoka, Takuya
N1 - Funding Information:
This research was supported by the Advanced Low Carbon Technology Research and Development Program from Japan Science and Technology Agency (T.K.) and by a Research Fellowship for Young Scientists from Japan Society for the Promotion of Science (K.K.). The authors are grateful to Dr. Yumi Fukunaga at the Ultramicroscopy Research Center, Kyushu University, for providing technical assistance in sample preparation for SEM analysis. N.H. is grateful to the Ministry of Education and Culture, Republic of Indonesia, for partial financial support through an Unggulan Scholarship. N.H is also grateful to Dian Anggraini, from Forest Products Research and Development Center for providing the picture of oil palm empty fruit bunch fibers.
Publisher Copyright:
© 2019 North Carolina State University.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.
AB - Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.
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M3 - Article
AN - SCOPUS:85076493531
VL - 14
SP - 6936
EP - 6957
JO - BioResources
JF - BioResources
SN - 1930-2126
IS - 3
ER -