Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications

Novitri Hastuti, Rizki Fitria Darmayanti, Safrina Dyah Hardiningtyas, Kyohei Kanomata, Kenji Sonomoto, Masahiro Goto, Takuya Kitaoka

Research output: Contribution to journalArticle

Abstract

Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.

Original languageEnglish
Pages (from-to)6936-6957
Number of pages22
JournalBioResources
Volume14
Issue number3
DOIs
Publication statusPublished - Aug 1 2019

Fingerprint

oxidized cellulose
Butanols
Palm oil
bioenergy
Butenes
Fermentation
fermentation
Nanofibers
cellulose
Biomass
Cellulose
oil
biomass
alginate
acetone
Alginate
Acetone
ethanol
glucose
Glucose

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Cite this

Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications. / Hastuti, Novitri; Darmayanti, Rizki Fitria; Hardiningtyas, Safrina Dyah; Kanomata, Kyohei; Sonomoto, Kenji; Goto, Masahiro; Kitaoka, Takuya.

In: BioResources, Vol. 14, No. 3, 01.08.2019, p. 6936-6957.

Research output: Contribution to journalArticle

Hastuti, Novitri ; Darmayanti, Rizki Fitria ; Hardiningtyas, Safrina Dyah ; Kanomata, Kyohei ; Sonomoto, Kenji ; Goto, Masahiro ; Kitaoka, Takuya. / Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications. In: BioResources. 2019 ; Vol. 14, No. 3. pp. 6936-6957.
@article{65c46e200d5240198260eb39360067e4,
title = "Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications",
abstract = "Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.",
author = "Novitri Hastuti and Darmayanti, {Rizki Fitria} and Hardiningtyas, {Safrina Dyah} and Kyohei Kanomata and Kenji Sonomoto and Masahiro Goto and Takuya Kitaoka",
year = "2019",
month = "8",
day = "1",
doi = "10.1021/bm0497769",
language = "English",
volume = "14",
pages = "6936--6957",
journal = "BioResources",
issn = "1930-2126",
publisher = "North Carolina University",
number = "3",

}

TY - JOUR

T1 - Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications

AU - Hastuti, Novitri

AU - Darmayanti, Rizki Fitria

AU - Hardiningtyas, Safrina Dyah

AU - Kanomata, Kyohei

AU - Sonomoto, Kenji

AU - Goto, Masahiro

AU - Kitaoka, Takuya

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.

AB - Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.

UR - http://www.scopus.com/inward/record.url?scp=85076493531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85076493531&partnerID=8YFLogxK

U2 - 10.1021/bm0497769

DO - 10.1021/bm0497769

M3 - Article

AN - SCOPUS:85076493531

VL - 14

SP - 6936

EP - 6957

JO - BioResources

JF - BioResources

SN - 1930-2126

IS - 3

ER -