Abstract
Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.
| Original language | English |
|---|---|
| Pages (from-to) | 6936-6957 |
| Number of pages | 22 |
| Journal | BioResources |
| Volume | 14 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 1 2019 |
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All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Bioengineering
- Waste Management and Disposal
Cite this
Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications. / Hastuti, Novitri; Darmayanti, Rizki Fitria; Hardiningtyas, Safrina Dyah; Kanomata, Kyohei; Sonomoto, Kenji; Goto, Masahiro; Kitaoka, Takuya.
In: BioResources, Vol. 14, No. 3, 01.08.2019, p. 6936-6957.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications
AU - Hastuti, Novitri
AU - Darmayanti, Rizki Fitria
AU - Hardiningtyas, Safrina Dyah
AU - Kanomata, Kyohei
AU - Sonomoto, Kenji
AU - Goto, Masahiro
AU - Kitaoka, Takuya
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.
AB - Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.
UR - http://www.scopus.com/inward/record.url?scp=85076493531&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076493531&partnerID=8YFLogxK
U2 - 10.1021/bm0497769
DO - 10.1021/bm0497769
M3 - Article
AN - SCOPUS:85076493531
VL - 14
SP - 6936
EP - 6957
JO - BioResources
JF - BioResources
SN - 1930-2126
IS - 3
ER -