Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications

Novitri Hastuti, Rizki Fitria Darmayanti, Safrina Dyah Hardiningtyas, Kyohei Kanomata, Kenji Sonomoto, Masahiro Goto, Takuya Kitaoka

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone-butanol-ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.

Original languageEnglish
Pages (from-to)6936-6957
Number of pages22
JournalBioResources
Volume14
Issue number3
Publication statusPublished - Aug 1 2019

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

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