Nanopillar, nanoball, and nanofibers for highly efficient analysis of biomolecules

Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalReview article

50 Citations (Scopus)

Abstract

DNA separation technologies combined with micro- and nanofabrication technologies found a breakthrough in genotyping and DNA sequencing. This tutorial review outlines the fabrication technologies for nano-scaled structures inside microchannels and how the precisely designed structures contribute to obtaining higher performances in DNA separations from the viewpoint of the fabrication process, "top-down" nanofabrication and "bottom-up" molecular self-assembly approaches. It was found that these nanofabricated structures generated the unique separation modes that could not be achieved by random-sized pores of conventional gel or polymer systems. Furthermore, it was found that nanoscale-specific phenomena such as electroosmotic flow should be taken into consideration for further development of nanofabricated structures in DNA analysis. These separation technologies will contribute as a core technology for a future integrated biomolecule anlaysis chip.

Original languageEnglish
Pages (from-to)948-956
Number of pages9
JournalChemical Society Reviews
Volume39
Issue number3
DOIs
Publication statusPublished - Mar 1 2010
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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