Nanospheres for DNA separation chips

Mari Tabuchi, Masanori Ueda, Noritada Kaji, Yuichi Yamasaki, Yukio Nagasaki, Kenichi Yoshikawa, Kazunori Kataoka, Yoshinobu Baba

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

We report here a technology to carry out separations of a wide range of DNA fragments with high speed and high resolution. The approach uses a nanoparticle medium, core-shell type nanospheres, in conjunction with a pressurization technique during microchip electrophoresis. DNA fragments up to 15 kilobase pairs (kbp) were successfully analyzed within 100 s without observing any saturation in migration rates. DNA fragments migrate in the medium while maintaining their characteristic molecular structure. To guarantee effective DNA loading and electrofocusing in the nanosphere solution, we developed a double pressurization technique. Optimal pressure conditions and concentrations of packed nanospheres are critical to achieve improved DNA separations.

Original languageEnglish
Pages (from-to)337-340
Number of pages4
JournalNature Biotechnology
Volume22
Issue number3
DOIs
Publication statusPublished - Mar 1 2004
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Tabuchi, M., Ueda, M., Kaji, N., Yamasaki, Y., Nagasaki, Y., Yoshikawa, K., ... Baba, Y. (2004). Nanospheres for DNA separation chips. Nature Biotechnology, 22(3), 337-340. https://doi.org/10.1038/nbt939