Nanospheres for DNA separation chips

Mari Tabuchi; Masanori Ueda; Noritada Kaji; Yuichi Yamasaki; Yukio Nagasaki; Kenichi Yoshikawa; Kazunori Kataoka; Yoshinobu Baba

doi:10.1038/nbt939

Nanospheres for DNA separation chips

Mari Tabuchi, Masanori Ueda, Noritada Kaji, Yuichi Yamasaki, Yukio Nagasaki, Kenichi Yoshikawa, Kazunori Kataoka, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

121 Citations (Scopus)

Abstract

We report here a technology to carry out separations of a wide range of DNA fragments with high speed and high resolution. The approach uses a nanoparticle medium, core-shell type nanospheres, in conjunction with a pressurization technique during microchip electrophoresis. DNA fragments up to 15 kilobase pairs (kbp) were successfully analyzed within 100 s without observing any saturation in migration rates. DNA fragments migrate in the medium while maintaining their characteristic molecular structure. To guarantee effective DNA loading and electrofocusing in the nanosphere solution, we developed a double pressurization technique. Optimal pressure conditions and concentrations of packed nanospheres are critical to achieve improved DNA separations.

Original language	English
Pages (from-to)	337-340
Number of pages	4
Journal	Nature Biotechnology
Volume	22
Issue number	3
DOIs	https://doi.org/10.1038/nbt939
Publication status	Published - Mar 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

Access to Document

10.1038/nbt939

Cite this

@article{a9db1fc7f49c4b82a419e7a3d8ecee3a,

title = "Nanospheres for DNA separation chips",

abstract = "We report here a technology to carry out separations of a wide range of DNA fragments with high speed and high resolution. The approach uses a nanoparticle medium, core-shell type nanospheres, in conjunction with a pressurization technique during microchip electrophoresis. DNA fragments up to 15 kilobase pairs (kbp) were successfully analyzed within 100 s without observing any saturation in migration rates. DNA fragments migrate in the medium while maintaining their characteristic molecular structure. To guarantee effective DNA loading and electrofocusing in the nanosphere solution, we developed a double pressurization technique. Optimal pressure conditions and concentrations of packed nanospheres are critical to achieve improved DNA separations.",

author = "Mari Tabuchi and Masanori Ueda and Noritada Kaji and Yuichi Yamasaki and Yukio Nagasaki and Kenichi Yoshikawa and Kazunori Kataoka and Yoshinobu Baba",

note = "Funding Information: This work was partially supported by a grant funding Core Research for Evolutionary Science and Technology from the Japan Science and Technology Agency, a grant from the New Energy and Industrial Technology Development Organization of the Ministry of Economy, Trade and Industry, Japan, a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Technology, Japan, and the 21st Century COE Program, Human Nutritional Science on Stress Control, Tokushima, Japan. We thank Chie Kuwahara, Ryosuka Kodaka and Fumiko Aboshi of the Tokyo University of Science and Eduardo Jule of the University of Tokyo for preparing the nanospheres. The authors would like to thank Tomoaki Hino, Ken Hirano, Fung Xu, Mohammad Jabasini, Hideya Nagata, Yasuko Tanaka and Emi Endo of the University of Tokushima for technical and secretarial assistance.",

year = "2004",

month = mar,

doi = "10.1038/nbt939",

language = "English",

volume = "22",

pages = "337--340",

journal = "Bio/Technology",

issn = "1087-0156",

publisher = "Nature Publishing Group",

number = "3",

}

TY - JOUR

T1 - Nanospheres for DNA separation chips

AU - Tabuchi, Mari

AU - Ueda, Masanori

AU - Kaji, Noritada

AU - Yamasaki, Yuichi

AU - Nagasaki, Yukio

AU - Yoshikawa, Kenichi

AU - Kataoka, Kazunori

AU - Baba, Yoshinobu

N1 - Funding Information: This work was partially supported by a grant funding Core Research for Evolutionary Science and Technology from the Japan Science and Technology Agency, a grant from the New Energy and Industrial Technology Development Organization of the Ministry of Economy, Trade and Industry, Japan, a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Technology, Japan, and the 21st Century COE Program, Human Nutritional Science on Stress Control, Tokushima, Japan. We thank Chie Kuwahara, Ryosuka Kodaka and Fumiko Aboshi of the Tokyo University of Science and Eduardo Jule of the University of Tokyo for preparing the nanospheres. The authors would like to thank Tomoaki Hino, Ken Hirano, Fung Xu, Mohammad Jabasini, Hideya Nagata, Yasuko Tanaka and Emi Endo of the University of Tokushima for technical and secretarial assistance.

PY - 2004/3

Y1 - 2004/3

N2 - We report here a technology to carry out separations of a wide range of DNA fragments with high speed and high resolution. The approach uses a nanoparticle medium, core-shell type nanospheres, in conjunction with a pressurization technique during microchip electrophoresis. DNA fragments up to 15 kilobase pairs (kbp) were successfully analyzed within 100 s without observing any saturation in migration rates. DNA fragments migrate in the medium while maintaining their characteristic molecular structure. To guarantee effective DNA loading and electrofocusing in the nanosphere solution, we developed a double pressurization technique. Optimal pressure conditions and concentrations of packed nanospheres are critical to achieve improved DNA separations.

AB - We report here a technology to carry out separations of a wide range of DNA fragments with high speed and high resolution. The approach uses a nanoparticle medium, core-shell type nanospheres, in conjunction with a pressurization technique during microchip electrophoresis. DNA fragments up to 15 kilobase pairs (kbp) were successfully analyzed within 100 s without observing any saturation in migration rates. DNA fragments migrate in the medium while maintaining their characteristic molecular structure. To guarantee effective DNA loading and electrofocusing in the nanosphere solution, we developed a double pressurization technique. Optimal pressure conditions and concentrations of packed nanospheres are critical to achieve improved DNA separations.

UR - http://www.scopus.com/inward/record.url?scp=1542276724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542276724&partnerID=8YFLogxK

U2 - 10.1038/nbt939

DO - 10.1038/nbt939

M3 - Article

C2 - 14990956

AN - SCOPUS:1542276724

SN - 1087-0156

VL - 22

SP - 337

EP - 340

JO - Bio/Technology

JF - Bio/Technology

IS - 3

ER -

Nanospheres for DNA separation chips

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this